Enzymatic glucose sensor based on Au nanoparticle and plant-like ZnO film modified electrode

A novel electrochemical glucose sensor was developed by employing a composite film of plant-like Zinc oxide (ZnO) and chitosan stabilized spherical gold nanoparticles (AuNPs) on which Glucose oxidaze (GOx) was immobilized. The ZnO was deposited on an indium tin oxide (ITO) coated glass and the AuNPs...

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Bibliographic Details
Published in:Materials Science & Engineering C 2015-01, Vol.46, p.548-552
Main Authors: Tian, Kun, Alex, Saji, Siegel, Gene, Tiwari, Ashutosh
Format: Article
Language:English
Subjects:
AuNPs
Dielectric Spectroscopy
Electrochemical impedance spectroscopy
Electrochemical properties
Electrodes
Glucose
Glucose - analysis
Glucose sensors
Gold
Gold - chemistry
Indium tin oxide
Microscopy, Electron, Transmission
Nanoparticles
Nanostructure
Sensors
Voltammetry
Zinc oxide
Zinc Oxide - chemistry
ZnO
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Summary:A novel electrochemical glucose sensor was developed by employing a composite film of plant-like Zinc oxide (ZnO) and chitosan stabilized spherical gold nanoparticles (AuNPs) on which Glucose oxidaze (GOx) was immobilized. The ZnO was deposited on an indium tin oxide (ITO) coated glass and the AuNPs of average diameter of 23nm were loaded on ZnO as the second layer. The prepared ITO/ZnO/AuNPs/GOx bioelectrode exhibited a low value of Michaelis–Menten constant of 1.70mM indicating a good bio-matrix for GOx. The studies of electrochemical properties of the electrode using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) showed that, the presence of AuNPs provides significant enhancement of the electron transfer rate during redox reactions. The linear sweep voltammetry (LSV) shows that the ITO/ZnO/AuNPs/GOx based sensor has a high sensitivity of 3.12μA·mM−1·cm−2 in the range of 50mg/dL to 400mg/dL glucose concentration. The results show promising application of the gold nanoparticle modified plant-like ZnO composite bioelectrode for electrochemical sensing of glucose.
ISSN:0928-4931
1873-0191
DOI:10.1016/j.msec.2014.10.064