In vitro biocompatibility of polypyrrole/PLGA conductive nanofiber scaffold with cultured rat hepatocytes

To intruduce conductive biomaterial into liver tissue engineering, a conductive nanofiber scaffold, polypyrrole/poly(lactic-co-glycolic)acid(PLGA), was designed and prepared via electro-spinning and oxidative polymerization. Effects of the scaffold on hepatocyte adhesion, viability and function were...

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Bibliographic Details
Published in:Materials research express 2014-09, Vol.1 (3), p.35402-9
Main Authors: Chu, Xue-Hui, Xu, Qian, Feng, Zhang-Qi, Xiao, Jiang-Qiang, Li, Qiang, Sun, Xi-Tai, Cao, Yang, Ding, Yi-Tao
Format: Article
Language:English
Subjects:
Adhesion
biocompatibility
hepatocyte
Liver
Membranes
nanofiber scaffold
Nanostructure
Polymerization
polypyrrole
Polypyrroles
Scaffolds
Secretions
Tissue engineering
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Summary:To intruduce conductive biomaterial into liver tissue engineering, a conductive nanofiber scaffold, polypyrrole/poly(lactic-co-glycolic)acid(PLGA), was designed and prepared via electro-spinning and oxidative polymerization. Effects of the scaffold on hepatocyte adhesion, viability and function were then investigated. SEM revealed pseudopodium formation and abundant extracellular matrix on the surface of PLGA membrane and polypyrrole/PLGA membrane. The adhesion rate, cellular activity, urea synthesis and albumin secretion of the hepatocytes cultured on polypyrrole/PLGA group were similar to those on the PLGA group, but were significantly higher than those on the control group. There were no significant differences in concentrations of LDH and TNF-α among three groups. These results suggested the potential application of this conductive nanofiber scaffold as a suitable substratum for hepatocyte culturing in liver tissue engineering.
ISSN:2053-1591
2053-1591
DOI:10.1088/2053-1591/1/3/035402