new virus-induced gene silencing vector based on Euphorbia mosaic virus-Yucatan peninsula for NPR1 silencing in Nicotiana benthamiana and Capsicum annuum var. Anaheim

Virus-induced gene silencing is based on the sequence-specific degradation of RNA. Here, a gene silencing vector derived from EuMV-YP, named pEuMV-YP:ΔAV1, was used to silence ChlI and NPR1 genes in Nicotiana benthamiana. The silencing of the ChlI transcripts was efficient in the stems, petioles and...

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Bibliographic Details
Published in:Biotechnology letters 2013-05, Vol.35 (5), p.811-823
Main Authors: Villanueva-Alonzo, Hernan J, Us-Camas, Rosa Y, López-Ochoa, Luisa A, Robertson, Dominique, Guerra-Peraza, Orlene, Minero-García, Yereni, Moreno-Valenzuela, Oscar A
Format: Article
Language:English
Subjects:
Applied Microbiology
Biochemistry
Biomedical and Life Sciences
Biotechnology
Bleaching
Capsicum - genetics
Capsicum - metabolism
Capsicum - virology
Capsicum annuum
Disease Resistance - genetics
Euphorbia
flowers
fruits
Gene Silencing
Genes
Genes, Plant - genetics
Genetic Vectors - genetics
Genomics
Leaves
Life Sciences
Mathematical analysis
Microbiology
Mosaic Viruses - genetics
Nicotiana - genetics
Nicotiana - metabolism
Nicotiana - virology
Nicotiana benthamiana
Original Research Paper
petioles
Plant biology
Plant Diseases
Plant Proteins - analysis
Plant Proteins - genetics
Plant Proteins - metabolism
Plants (organisms)
Plasmids
RNA
Stems
VAR
Vectors (mathematics)
Viruses
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Description
Summary:Virus-induced gene silencing is based on the sequence-specific degradation of RNA. Here, a gene silencing vector derived from EuMV-YP, named pEuMV-YP:ΔAV1, was used to silence ChlI and NPR1 genes in Nicotiana benthamiana. The silencing of the ChlI transcripts was efficient in the stems, petioles and leaves as reflected in tissue bleaching and reduced transcript levels. The silencing was stable, reaching the flowers and fruits, and was observed throughout the life cycle of the plants. Additionally, the silencing of the NPR1 gene was efficient in both N. benthamiana and Capsicum annuum. After silencing, the plants’ viral symptoms increased to levels similar to those seen in wild-type plants. These results suggest that NPR1 plays a role in the compatible interactions of EuMV-YP N. benthamiana and EuMV-C. annum var. anaheim.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-013-1146-1