Scale-up of human embryonic stem cell culture using a hollow fibre bioreactor

The commercialisation of human embryonic stem cell derived cell therapies for large patient populations is reliant on both minimising expensive and variable manual-handling methods whilst realising economies of scale. The Quantum Cell Expansion System, a hollow fibre bioreactor (Terumo BCT), was use...

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Bibliographic Details
Published in:Biotechnology letters 2012-12, Vol.34 (12), p.2307-2315
Main Authors: Roberts, Iwan, Baila, Stefano, Rice, R. Brent, Janssens, Michiel Etienne, Nguyen, Kim, Moens, Nathalie, Ruban, Ludmila, Hernandez, Diana, Coffey, Pete, Mason, Chris
Format: Article
Language:English
Subjects:
Applied Microbiology
Biochemistry
Bioengineering
Biological and medical sciences
Biomedical and Life Sciences
Bioreactors
Biotechnology
cell culture
Cell Culture Techniques - methods
Cellular biology
Culture
economies of scale
embryonic stem cells
Embryonic Stem Cells - physiology
Embryos
Fibre
Fundamental and applied biological sciences. Psychology
Gene Expression
Human
Humans
Life Sciences
Markers
Methods. Procedures. Technologies
Microbiology
Original Research Paper
Pilots
Stage-Specific Embryonic Antigens - biosynthesis
Stem cells
Various methods and equipments
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Summary:The commercialisation of human embryonic stem cell derived cell therapies for large patient populations is reliant on both minimising expensive and variable manual-handling methods whilst realising economies of scale. The Quantum Cell Expansion System, a hollow fibre bioreactor (Terumo BCT), was used in a pilot study to expand 60 million human embryonic stem cells to 708 million cells. Further improvements can be expected with optimisation of media flow rates throughout the run to better control the cellular microenvironment. High levels of pluripotency marker expression were maintained on the bioreactor, with 97.7 % of cells expressing SSEA-4 when harvested.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-012-1033-1