Acceleration effect of amino acid supplementation on glycerol assimilation by Escherichia coli in minimal medium

Growth of Escherichia coli BL21 in a glycerol minimal medium was accelerated following supplementation with trace amounts of amino acid (0.35 mM). Of 12 amino acids tested, Arg and Ser gave the highest response, increasing cell growth by 63 and 53 %, respectively, compared to control cells. The abil...

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Bibliographic Details
Published in:Biotechnology letters 2013-09, Vol.35 (9), p.1495-1500
Main Authors: Xiang, Gang, Li, Jiang, Duan, JiFu, Shao, Fei, Xu, Jiajie, Fu, Shuilin, Gong, Heng
Format: Article
Language:English
Subjects:
Acceleration
Amino acids
Amino Acids - metabolism
Applied Microbiology
Assimilation
Biochemistry
Biomedical and Life Sciences
Biotechnology
Carbon
Carbon sources
Cell growth
Culture Media - chemistry
E coli
Escherichia coli
Escherichia coli - growth & development
Escherichia coli - metabolism
glycerol
Glycerol - metabolism
Glycerols
lactose
Life Sciences
Microbiology
Original Research Paper
Utilization
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Summary:Growth of Escherichia coli BL21 in a glycerol minimal medium was accelerated following supplementation with trace amounts of amino acid (0.35 mM). Of 12 amino acids tested, Arg and Ser gave the highest response, increasing cell growth by 63 and 53 %, respectively, compared to control cells. The ability of amino acids to accelerate cell growth was “switch-like” and was achieved by promoting glycerol utilization, which may be applied to shorten the long lag-phase when glycerol is used as carbon source. Acceleration of cell growth following amino acid supplementation was also observed using lactose minimal medium.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-013-1232-4