Dynamic sieving capillary electrophoresis analysis of xylitol selenite-induced apoptosis in SMMC-7221 cells

DNA ladder fragments, regarded as a biochemical hallmark of apoptosis, have been separated quickly and successfully by capillary electrophoresis. Inter-nucleosomal DNA fragmentations induced by xylitol selenite were determined for the first time, while hydroxypropylmethylcellulose (HPMC) was served...

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Bibliographic Details
Published in:Biotechnology letters 2012-09, Vol.34 (9), p.1617-1621
Main Authors: Lei, Ming, Chen, Didi, Deng, Xiaojie, Liu, Jing, Chen, Layue, Liu, Yanli, Li, Bing, Yao, Hancao, Xiong, Guomei, Cao, Yu, Yang, Jihong, Qi, Chao
Format: Article
Language:English
Subjects:
Animal models
anticarcinogenic activity
Apoptosis
Apoptosis - drug effects
Applied Microbiology
Biochemistry
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Cancer
Capillarity
capillary electrophoresis
Carcinogenesis
Cell Line, Tumor
Deoxyribonucleic acid
Disease prevention
DNA
DNA Fragmentation
Dynamics
Electrophoresis
Electrophoresis, Capillary - methods
Filtration - methods
Fragments
Fundamental and applied biological sciences. Psychology
gel electrophoresis
Hepatocytes - drug effects
Humans
Hypromellose Derivatives
Life Sciences
Methylcellulose - analogs & derivatives
Methylcellulose - chemistry
Microbiology
nucleosomes
Original Research Paper
Selenites
Selenium
Selenium compounds
sieving
Sodium Selenite - toxicity
Xylitol
Xylitol - toxicity
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Summary:DNA ladder fragments, regarded as a biochemical hallmark of apoptosis, have been separated quickly and successfully by capillary electrophoresis. Inter-nucleosomal DNA fragmentations induced by xylitol selenite were determined for the first time, while hydroxypropylmethylcellulose (HPMC) was served as the sieving matrix in dynamic sieving capillary electrophoresis. The calibration curve (r2 = 0.991) was established and multiples of two different nucleosomes (140 and 180 bp) were formed in the presence of xylitol selenite. Selenium compounds inhibited carcinogenesis in animal models, SMMC-7221 cells and several other cells by increasing apoptosis. The described method was useful in elucidating the anticancer activities of xylitol selenite and other selenium compounds, which was more effective to detect small fragments than slab gel electrophoresis.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-012-0945-0