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Effect of zein on biodegradable inserts for the delivery of tetracycline within periodontal pockets
Treatment with antibiotics within the periodontal pocket against bacterial infections represents a useful and adjunctive tool to conventional therapy for healing and teeth preservation. With this function in view, an implantable, tetracycline delivery device for the treatment of periodontal disease...
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Published in: | Journal of biomaterials applications 2012-08, Vol.27 (2), p.187-200 |
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description | Treatment with antibiotics within the periodontal pocket against bacterial infections represents a useful and adjunctive tool to conventional therapy for healing and teeth preservation. With this function in view, an implantable, tetracycline delivery device for the treatment of periodontal disease was developed. The aim of this study was to develop biodegradable, tetracycline-loaded microparticles made of two polymers: PLGA and zein which were compressed into monolithic devices. In this polymer delivery system, the encapsulation efficiency, release characteristics, drug–polymer interaction, and antibacterial activity of loaded drug were investigated. The interaction of tetracycline with the corn protein zein was studied by nuclear magnetic resonance (NMR), Fourier transform infrared, and X-ray diffraction. The hydrophobic interaction of tetracycline with zein in the formulations was deduced from the NMR studies, whereas X-ray diffraction studies showed a new crystalline state of the drug in the presence of the protein. Zein was not denatured by preparation of inserts. Sustained release of tetracycline was obtained, and the proportion of zein in the inserts had a great impact on the drug release. Finally, an effective tetracycline release from inserts against Staphylococcus aureus was achieved over 30 days. In conclusion, the PLGA:zein delivery system described in this study was found to be effective in controlled delivery of tetracycline, and hence may be suitable for intra-pocket delivery of antimicrobial agents in the treatment of periodontitis. |
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With this function in view, an implantable, tetracycline delivery device for the treatment of periodontal disease was developed. The aim of this study was to develop biodegradable, tetracycline-loaded microparticles made of two polymers: PLGA and zein which were compressed into monolithic devices. In this polymer delivery system, the encapsulation efficiency, release characteristics, drug–polymer interaction, and antibacterial activity of loaded drug were investigated. The interaction of tetracycline with the corn protein zein was studied by nuclear magnetic resonance (NMR), Fourier transform infrared, and X-ray diffraction. The hydrophobic interaction of tetracycline with zein in the formulations was deduced from the NMR studies, whereas X-ray diffraction studies showed a new crystalline state of the drug in the presence of the protein. Zein was not denatured by preparation of inserts. Sustained release of tetracycline was obtained, and the proportion of zein in the inserts had a great impact on the drug release. Finally, an effective tetracycline release from inserts against Staphylococcus aureus was achieved over 30 days. In conclusion, the PLGA:zein delivery system described in this study was found to be effective in controlled delivery of tetracycline, and hence may be suitable for intra-pocket delivery of antimicrobial agents in the treatment of periodontitis.</description><identifier>ISSN: 0885-3282</identifier><identifier>EISSN: 1530-8022</identifier><identifier>DOI: 10.1177/0885328211398968</identifier><identifier>PMID: 21586598</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Absorbable Implants ; Anti-Bacterial Agents - administration & dosage ; Anti-Bacterial Agents - pharmacology ; Bacteria ; Biodegradability ; Diffraction ; Drug Delivery Systems - instrumentation ; Drugs ; Humans ; Inserts ; Lactic Acid - chemistry ; Nuclear magnetic resonance ; Nuclear Magnetic Resonance, Biomolecular ; Periodontal Pocket - drug therapy ; Periodontal Pocket - microbiology ; Polyglycolic Acid - chemistry ; Staphylococcal Infections - drug therapy ; Staphylococcus aureus ; Staphylococcus aureus - drug effects ; Tetracycline - administration & dosage ; Tetracycline - pharmacology ; Tetracyclines ; X-Ray Diffraction ; Zea mays - chemistry ; Zein ; Zein - chemistry</subject><ispartof>Journal of biomaterials applications, 2012-08, Vol.27 (2), p.187-200</ispartof><rights>The Author(s) 2011 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c403t-18c61131ccabef58d610a9e807c5403b264d8801391cef83a5f625e30c23e89a3</citedby><cites>FETCH-LOGICAL-c403t-18c61131ccabef58d610a9e807c5403b264d8801391cef83a5f625e30c23e89a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925,79364</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21586598$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Sousa, Fábio Oliveira</creatorcontrib><creatorcontrib>Blanco-Méndez, Jose</creatorcontrib><creatorcontrib>Pérez-Estévez, Antonio</creatorcontrib><creatorcontrib>Seoane-Prado, Rafael</creatorcontrib><creatorcontrib>Luzardo-Álvarez, Asteria</creatorcontrib><title>Effect of zein on biodegradable inserts for the delivery of tetracycline within periodontal pockets</title><title>Journal of biomaterials applications</title><addtitle>J Biomater Appl</addtitle><description>Treatment with antibiotics within the periodontal pocket against bacterial infections represents a useful and adjunctive tool to conventional therapy for healing and teeth preservation. With this function in view, an implantable, tetracycline delivery device for the treatment of periodontal disease was developed. The aim of this study was to develop biodegradable, tetracycline-loaded microparticles made of two polymers: PLGA and zein which were compressed into monolithic devices. In this polymer delivery system, the encapsulation efficiency, release characteristics, drug–polymer interaction, and antibacterial activity of loaded drug were investigated. The interaction of tetracycline with the corn protein zein was studied by nuclear magnetic resonance (NMR), Fourier transform infrared, and X-ray diffraction. The hydrophobic interaction of tetracycline with zein in the formulations was deduced from the NMR studies, whereas X-ray diffraction studies showed a new crystalline state of the drug in the presence of the protein. Zein was not denatured by preparation of inserts. Sustained release of tetracycline was obtained, and the proportion of zein in the inserts had a great impact on the drug release. Finally, an effective tetracycline release from inserts against Staphylococcus aureus was achieved over 30 days. In conclusion, the PLGA:zein delivery system described in this study was found to be effective in controlled delivery of tetracycline, and hence may be suitable for intra-pocket delivery of antimicrobial agents in the treatment of periodontitis.</description><subject>Absorbable Implants</subject><subject>Anti-Bacterial Agents - administration & dosage</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Bacteria</subject><subject>Biodegradability</subject><subject>Diffraction</subject><subject>Drug Delivery Systems - instrumentation</subject><subject>Drugs</subject><subject>Humans</subject><subject>Inserts</subject><subject>Lactic Acid - chemistry</subject><subject>Nuclear magnetic resonance</subject><subject>Nuclear Magnetic Resonance, Biomolecular</subject><subject>Periodontal Pocket - drug therapy</subject><subject>Periodontal Pocket - microbiology</subject><subject>Polyglycolic Acid - chemistry</subject><subject>Staphylococcal Infections - drug therapy</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Tetracycline - administration & dosage</subject><subject>Tetracycline - pharmacology</subject><subject>Tetracyclines</subject><subject>X-Ray Diffraction</subject><subject>Zea mays - chemistry</subject><subject>Zein</subject><subject>Zein - chemistry</subject><issn>0885-3282</issn><issn>1530-8022</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNqNkTtPxDAQhC0EguPRUyGXNAE_YmdTIsRLQqKBOnKc9RHIxYftAx2_Hp8OKJAQVFvMNyPNDiGHnJ1wXlWnDEBJAYJzWUOtYYNMuJKsACbEJpms5GKl75DdGJ8YY6ou9TbZEVyBVjVMiL1wDm2i3tF37EfqR9r2vsNpMJ1pB6T9GDGkSJ0PND0i7XDoXzEsV46EKRi7tEM_In3r02MOmGPIfj8mM9C5t8-Y4j7ZcmaIePB598jD5cX9-XVxe3d1c352W9iSyVRwsDoX4daaFp2CTnNmagRWWZWBVuiyA2C5KrfoQBrltFAomRUSoTZyjxyvc-fBvywwpmbWR4vDYEb0i9hwDQq4KlX5N8ry22pdVv9CmSxBVSKjbI3a4GMM6Jp56GcmLDPUrAZrfg6WLUef6Yt2ht234WuhDBRrIJopNk9-Ecb8w98DPwBjUpzn</recordid><startdate>201208</startdate><enddate>201208</enddate><creator>de Sousa, Fábio Oliveira</creator><creator>Blanco-Méndez, Jose</creator><creator>Pérez-Estévez, Antonio</creator><creator>Seoane-Prado, Rafael</creator><creator>Luzardo-Álvarez, Asteria</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>F28</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>201208</creationdate><title>Effect of zein on biodegradable inserts for the delivery of tetracycline within periodontal pockets</title><author>de Sousa, Fábio Oliveira ; Blanco-Méndez, Jose ; Pérez-Estévez, Antonio ; Seoane-Prado, Rafael ; Luzardo-Álvarez, Asteria</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c403t-18c61131ccabef58d610a9e807c5403b264d8801391cef83a5f625e30c23e89a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Absorbable Implants</topic><topic>Anti-Bacterial Agents - administration & dosage</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Bacteria</topic><topic>Biodegradability</topic><topic>Diffraction</topic><topic>Drug Delivery Systems - instrumentation</topic><topic>Drugs</topic><topic>Humans</topic><topic>Inserts</topic><topic>Lactic Acid - chemistry</topic><topic>Nuclear magnetic resonance</topic><topic>Nuclear Magnetic Resonance, Biomolecular</topic><topic>Periodontal Pocket - drug therapy</topic><topic>Periodontal Pocket - microbiology</topic><topic>Polyglycolic Acid - chemistry</topic><topic>Staphylococcal Infections - drug therapy</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Tetracycline - administration & dosage</topic><topic>Tetracycline - pharmacology</topic><topic>Tetracyclines</topic><topic>X-Ray Diffraction</topic><topic>Zea mays - chemistry</topic><topic>Zein</topic><topic>Zein - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Sousa, Fábio Oliveira</creatorcontrib><creatorcontrib>Blanco-Méndez, Jose</creatorcontrib><creatorcontrib>Pérez-Estévez, Antonio</creatorcontrib><creatorcontrib>Seoane-Prado, Rafael</creatorcontrib><creatorcontrib>Luzardo-Álvarez, Asteria</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Journal of biomaterials applications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Sousa, Fábio Oliveira</au><au>Blanco-Méndez, Jose</au><au>Pérez-Estévez, Antonio</au><au>Seoane-Prado, Rafael</au><au>Luzardo-Álvarez, Asteria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of zein on biodegradable inserts for the delivery of tetracycline within periodontal pockets</atitle><jtitle>Journal of biomaterials applications</jtitle><addtitle>J Biomater Appl</addtitle><date>2012-08</date><risdate>2012</risdate><volume>27</volume><issue>2</issue><spage>187</spage><epage>200</epage><pages>187-200</pages><issn>0885-3282</issn><eissn>1530-8022</eissn><abstract>Treatment with antibiotics within the periodontal pocket against bacterial infections represents a useful and adjunctive tool to conventional therapy for healing and teeth preservation. With this function in view, an implantable, tetracycline delivery device for the treatment of periodontal disease was developed. The aim of this study was to develop biodegradable, tetracycline-loaded microparticles made of two polymers: PLGA and zein which were compressed into monolithic devices. In this polymer delivery system, the encapsulation efficiency, release characteristics, drug–polymer interaction, and antibacterial activity of loaded drug were investigated. The interaction of tetracycline with the corn protein zein was studied by nuclear magnetic resonance (NMR), Fourier transform infrared, and X-ray diffraction. The hydrophobic interaction of tetracycline with zein in the formulations was deduced from the NMR studies, whereas X-ray diffraction studies showed a new crystalline state of the drug in the presence of the protein. Zein was not denatured by preparation of inserts. Sustained release of tetracycline was obtained, and the proportion of zein in the inserts had a great impact on the drug release. Finally, an effective tetracycline release from inserts against Staphylococcus aureus was achieved over 30 days. In conclusion, the PLGA:zein delivery system described in this study was found to be effective in controlled delivery of tetracycline, and hence may be suitable for intra-pocket delivery of antimicrobial agents in the treatment of periodontitis.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>21586598</pmid><doi>10.1177/0885328211398968</doi><tpages>14</tpages></addata></record> |
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subjects | Absorbable Implants Anti-Bacterial Agents - administration & dosage Anti-Bacterial Agents - pharmacology Bacteria Biodegradability Diffraction Drug Delivery Systems - instrumentation Drugs Humans Inserts Lactic Acid - chemistry Nuclear magnetic resonance Nuclear Magnetic Resonance, Biomolecular Periodontal Pocket - drug therapy Periodontal Pocket - microbiology Polyglycolic Acid - chemistry Staphylococcal Infections - drug therapy Staphylococcus aureus Staphylococcus aureus - drug effects Tetracycline - administration & dosage Tetracycline - pharmacology Tetracyclines X-Ray Diffraction Zea mays - chemistry Zein Zein - chemistry |
title | Effect of zein on biodegradable inserts for the delivery of tetracycline within periodontal pockets |
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