Recovery of absorbance spectra of micrometer-sized biological and inanimate particles

In this paper, we first provide an overview of the Mie type scattering at absorbing materials and existing correction methods, followed by a new method to obtain the pure absorbance spectra of biological systems with spherical symmetry. This method is a further development of the recently described...

Full description

Saved in:
Bibliographic Details
Published in:Analyst (London) 2015-05, Vol.140 (9), p.3273-3284
Main Authors: Lukacs, R, Blümel, R, Zimmerman, B, Bağcıoğlu, M, Kohler, A
Format: Article
Language:English
Subjects:
Absorbance
Absorption
Algorithms
Biological
Cupressaceae - chemistry
Light
Particle Size
Pollen
Pollen - chemistry
Polymethyl Methacrylate - chemistry
Refractive index
Refractometry
Scattering, Radiation
Spectra
Spectroscopy, Fourier Transform Infrared
Symmetry
Synchrotrons
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this paper, we first provide an overview of the Mie type scattering at absorbing materials and existing correction methods, followed by a new method to obtain the pure absorbance spectra of biological systems with spherical symmetry. This method is a further development of the recently described iterative algorithm of van Dijk et al. The method is tested on FTIR synchrotron spectra of polymethyl methacrylate (PMMA) microspheres and pollen grains with approximately spherical shape. The imaginary part of the refractive index was successfully recovered for both systems. Good agreement was obtained between the pure absorbance spectra obtained by this method and the measured spectra.
ISSN:0003-2654
1364-5528
DOI:10.1039/c5an00401b