novel perfused rotary bioreactor for cardiomyogenesis of embryonic stem cells

Developments in bioprocessing technology play an important role for overcoming challenges in cardiac tissue engineering. To this end, our laboratory has developed a novel rotary perfused bioreactor for supporting three-dimensional cardiac tissue engineering. The dynamic culture environments provided...

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Bibliographic Details
Published in:Biotechnology letters 2014-05, Vol.36 (5), p.947-960
Main Authors: Teo, Ailing, Mantalaris, Athanasios, Song, Kedong, Lim, Mayasari
Format: Article
Language:English
Subjects:
Animals
Applied Microbiology
Biochemistry
Biomedical and Life Sciences
bioprocessing
Bioreactors
Biotechnology
Cardiomyocytes
Cell Culture Techniques - instrumentation
Cell Culture Techniques - methods
Cell Differentiation - physiology
Construction
Culture
Dynamics
embryonic stem cells
Embryonic Stem Cells - cytology
Gene expression
Heart
Life Sciences
Mice
Microbiology
Myocytes, Cardiac - cytology
Original Research Paper
Stem cells
Three dimensional
tissue culture
Tissue engineering
troponin T
viability
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Summary:Developments in bioprocessing technology play an important role for overcoming challenges in cardiac tissue engineering. To this end, our laboratory has developed a novel rotary perfused bioreactor for supporting three-dimensional cardiac tissue engineering. The dynamic culture environments provided by our novel perfused rotary bioreactor and/or the high-aspect rotating vessel produced constructs with higher viability and significantly higher cell numbers (up to 4 × 10⁵ cells/bead) than static tissue culture flasks. Furthermore, cells in the perfused rotary bioreactor showed earlier gene expressions of cardiac troponin-T, α- and β-myosin heavy chains with higher percentages of cardiac troponin-I-positive cells and better uniformity of sacromeric α-actinin expression. A dynamic and perfused environment, as provided by this bioreactor, provides a superior culture performance in cardiac differentiation for embryonic stem cells particularly for larger 3D constructs.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-014-1456-y