Transgene-induced pleiotropic effects in transplastomic plants

Since the first demonstration of stable transgene integration in the plastid genome (plastome) of higher plants, plastid transformation has been used for a wide range of purposes, including basic studies as well as biotechnological applications, showing that transplastomic plants are an effective sy...

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Bibliographic Details
Published in:Biotechnology letters 2014-02, Vol.36 (2), p.229-239
Main Authors: Scotti, N, Cardi, T
Format: Article
Language:English
Subjects:
Applied Microbiology
Biochemistry
Biomedical and Life Sciences
Biotechnology
Chloroplasts
Chloroplasts - genetics
Chloroplasts - metabolism
Containment
Genetic transformation
Genomes
Life Sciences
Microbiology
Mutagenesis
Nuclear transformations
Plant Development
Plant growth
Plant Physiological Phenomena
Plants (organisms)
Plants, Genetically Modified - genetics
Plants, Genetically Modified - metabolism
plastid DNA
Plastids
Proteins
Recombinant
recombinant proteins
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Review
Studies
Transformations
Transgenes
Transgenic plants
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Summary:Since the first demonstration of stable transgene integration in the plastid genome (plastome) of higher plants, plastid transformation has been used for a wide range of purposes, including basic studies as well as biotechnological applications, showing that transplastomic plants are an effective system to produce recombinant proteins. Compared to nuclear transformation, the main advantages of this technology are the high and stable production level of proteins as well as the natural containment of transgenes. To date, more than 100 transgenes have been successfully expressed in plant chloroplasts. In some cases, however, unintended pleiotropic effects on plant growth and physiology were shown in transplastomic plants. In this paper, we review such effects and discuss some of the technologies developed to overcome them.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-013-1356-6