Over-expression of PsGPD, a mushroom glyceraldehyde-3-phosphate dehydrogenase gene, enhances salt tolerance in rice plants

Transgenic potatoes expressing glyceraldehyde-3-phosphate dehydrogenase (GPD), isolated from the oyster mushroom, Pleurotus sajor-caju, had increased tolerance to salt stress (Jeong et al. Biochem Biophys Res Commun 278:192–196, 2000). To examine the physiological mechanisms enhancing salt tolerance...

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Bibliographic Details
Published in:Biotechnology letters 2014-08, Vol.36 (8), p.1641-1648
Main Authors: Cho, Jung-Il, Lim, Hye-Min, Siddiqui, Zamin Shaheed, Park, Sung-Han, Kim, A-Ram, Kwon, Taek-Ryoun, Lee, Seong-Kon, Park, Soo-Chul, Jeong, Mi-Jeong, Lee, Gang-Seob
Format: Article
Language:English
Subjects:
Applied Microbiology
Biochemistry
Biomass
Biomedical and Life Sciences
Biotechnology
Cultivars
Darkness
Dehydrogenase
DNA, Bacterial - genetics
Flowers & plants
Gene expression
gene overexpression
genes
glyceraldehyde-3-phosphate dehydrogenase
Glyceraldehyde-3-Phosphate Dehydrogenases - genetics
Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism
Life Sciences
Microbiology
Moisture content
Mushrooms
Original Research Paper
Oryza - genetics
Oryza - physiology
Oryza sativa
Osmosis
osmotic pressure
Oysters
Phosphates
physiological response
Physiology
Plant Stomata - physiology
Plants (organisms)
Plants, Genetically Modified
Pleurotus - enzymology
Pleurotus - genetics
Pleurotus ostreatus
Pleurotus sajor-caju
Potatoes
Rice
Salt
salt stress
Salt tolerance
Solanum tuberosum
Stomatal conductance
Stress, Physiological
Tolerances
Transgenic
Transgenic plants
Water
Water content
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Summary:Transgenic potatoes expressing glyceraldehyde-3-phosphate dehydrogenase (GPD), isolated from the oyster mushroom, Pleurotus sajor-caju, had increased tolerance to salt stress (Jeong et al. Biochem Biophys Res Commun 278:192–196, 2000). To examine the physiological mechanisms enhancing salt tolerance in GPD-transgenic rice plants, the salt tolerance of five GPD transgenic rice lines (T1–T5) derived from Dongjin rice cultivar were evaluated in a fixed 150 mM saline environment in comparison to two known wild-type rice cultivars, Dongjin (salt sensitive) and Pokali (salt tolerant). Transgenic lines, T2, T3, and T5, had a substantial increase in biomass and relative water content compared to Dongjin. Stomatal conductance and osmotic potential were higher in the GPD transgenic lines and were similar to those in Pokali. The results are discussed based on the comparative physiological response of GPD transgenic lines with those of the salt-sensitive and salt-tolerant rice cultivars.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-014-1522-5