Hyper expression of an environmentally friendly synthetic polymer gene

Biodegradable polymers offer an environmentally friendly alternative to petroleum-based polymers. Applications of protein based polymers include the use of these compounds in the fields of medicine, molecular-based energy conversions, the manufacture of unique fibers, coatings and biodegradable plas...

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Bibliographic Details
Published in:Biotechnology letters 1995-07, Vol.17 (7), p.745-750
Main Authors: GUDA, C, ZHANG, X, MCPHERSON, D. T, XU, J, CHERRY, J. H, URRY, D. W, DANIELL, H
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Escherichia coli
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Methods. Procedures. Technologies
Modification of gene expression level
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Summary:Biodegradable polymers offer an environmentally friendly alternative to petroleum-based polymers. Applications of protein based polymers include the use of these compounds in the fields of medicine, molecular-based energy conversions, the manufacture of unique fibers, coatings and biodegradable plastics. We report here expression of a synthetic gene G-(VPGVG) sub(119)-VPGV coding for the EG-120mer (elastomer) in E. coli. Polymer expression is observed in uninduced cells grown in terrific broth in polyacrylamide gels negatively stained with CuCl sub(2). Electron micrographs reveal formation of inclusion bodies in uninduced cells occupying upto 80-90% of the cell volume under optimal growth conditions. To the best of our knowledge this report represents the first demonstration of hyper expression of a synthetic gene (with no natural analog) in E. coli.
ISSN:0141-5492
1573-6776
DOI:10.1007/BF00130362