Sensitive simultaneous determination of three sulfanilamide artificial sweeters by capillary electrophoresis with on-line preconcentration and contactless conductivity detection

• Published in: Food chemistry 2015-12, Vol.188, p.446-451
• Main Authors: Yang, Lirong, Zhou, ShengJi, Xiao, Yuezhou, Tang, Yufeng, Xie, Tianyao
• Format: Article
• Language: English
• Subjects: Acesulfame-K; Beverages - analysis; Beverages - standards; Capillary electrophoresis; Contactless conductivity detection; Cyclamates - analysis; Electrophoresis, Capillary - instrumentation; Electrophoresis, Capillary - methods; Limit of Detection; On-line preconcentration; Reproducibility of Results; Saccharin - analysis; Sodium cyclamate; Sodium saccharin; Sulfanilamides - analysis; Sweetening Agents - analysis
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2015.04.060

•A sensitive new method for determination of sulfanilamide artificial sweeteners is proposed.•The method is based on FASI–CE–C4D technique to achieve high sensitivity enhancement and lower LODs.•Simplicity, rapidity, low cost, high efficiency and easy sample treatment are some advantages of this method.•The method was validated for directly determination of artificial sweeteners in commercial beverages. A sensitive method followed by capillary electrophoresis with on-line perconcentration and capacitively coupled contactless conductivity detection (CE–C4D) was evaluated as a novel approach for the determination of three sulfanilamide artificial sweeteners (acesulfame-K, sodium saccharin and sodium cyclamate) in beverages. The on-line preconcentration technique, namely field-amplified sample injection, coupled with CE–C4D were successfully developed and optimized. The separation was achieved within 10min under the following conditions: an uncoated fused-silica capillary (45cm×50μm i.d., Leff=40cm), 20mmolL−1 HAc as running buffer, separation voltage of −12kV, electrokinetic injection of −11kV×8s. The detection limits of acesulfame-K, sodium saccharin and sodium cyclamate were 4.4, 6.7 and 8.8μgL−1, respectively. The relative standard deviation varied in the range of 3.0–5.0%. Results of this study show a great potential method for the fast screening of these artificial sweeteners contents in commercial beverages.