The tetraspanin gene MaPls1 contributes to virulence by affecting germination, appressorial function and enzymes for cuticle degradation in the entomopathogenic fungus, Metarhizium acridum

• Published in: Environmental microbiology 2013-11, Vol.15 (11), p.2966-2979
• Main Authors: Luo, Sha, He, Min, Cao, Yueqing, Xia, Yuxian
• Format: Article
• Language: English
• Subjects: Animal, plant and microbial ecology; Animals; Base Sequence; Biological and medical sciences; Calmodulin - metabolism; Cyclic AMP-Dependent Protein Kinases - metabolism; DNA, Fungal - analysis; DNA, Fungal - genetics; Fundamental and applied biological sciences. Psychology; Gene Deletion; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Fungal - genetics; General aspects; Germination; Insecta; Insecta - microbiology; Invertebrates; Kinases; Metarhizium; Metarhizium - genetics; Metarhizium - pathogenicity; Microbial ecology; Microbiology; Miscellaneous; Mycelium - metabolism; Mycology; Pathology; Sequence Analysis, DNA; Signal Transduction - genetics; Tetraspanins - genetics; Virulence - genetics; Virulence Factors - genetics
• ISSN: 1462-2912; 1462-2920
• DOI: 10.1111/1462-2920.12166

Summary In most eukaryotes, tetraspanins regulate cellular activities by associating with other membrane components. In phytopathogenic fungi, the tetraspanin Pls1 controls appressorium‐mediated penetration. However, regulation of Pls1 and its associated signalling pathways are not clear. In this study, the MaPls1 gene from the entomopathogenic fungus Metarhizium acridum was functionally characterized. MaPls1 was highly expressed in mycelium and appressorium, and accumulated on the plasma membrane or in the cytoplasm. Compared with a wild‐type strain, the deletion mutant ΔMaPls1 had delayed germination and appressorium formation and impaired turgor pressure on locust wings, but normal germination on medium and non‐host insect matrices. Bioassays showed that ΔMaPls1 had decreased virulence and hyphal body formation in haemolymph when topically inoculated, but was not different from wild type when the insect cuticle was bypassed. Moreover, the ability to grow out of the cuticle was impaired in ΔMaPls1. Digital gene expression profiling revealed that genes involved in hydrolysing host cuticle and cell wall synthesis and remodelling were downregulated in ΔMaPls1. MaPls1 participated in crosstalk with signalling pathways such as the cyclic adenosine monophosphate‐dependent protein kinase A and calmodulin‐dependent pathways. Taken together, these results demonstrated the important roles of MaPls1 at the early stage of infection‐associated development in M. acridum.