First evidence of molecular characterization of rohu carp Sox2 gene being expressed in proliferating spermatogonial cells

Because little is known about the function of Sox2 (Sry-related box-2) in teleosts, the objective of this study was to clone and characterize Sox2 complementary DNA (cDNA) from the testis of Indian major carp, Labeo rohita (rohu). The full-length cDNA contained an open reading frame of 936 nucleotid...

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Bibliographic Details
Published in:Theriogenology 2015-07, Vol.84 (2), p.268-276.e1
Main Authors: Patra, Swagat Kumar, Chakrapani, Vemulawada, Panda, Rudra Prasanna, Mohapatra, Chinmayee, Jayasankar, Pallipuram, Barman, Hirak Kumar
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Carps - metabolism
Cell Proliferation
Cloning, Molecular
DNA, Complementary - genetics
Fish Proteins - genetics
Gene Expression
High-mobility group box domain
Labeo rohita
Male
Molecular Sequence Data
Phylogeny
RNA, Messenger - analysis
Rohu
Sequence Analysis, DNA - veterinary
SOX Transcription Factors - analysis
SOX Transcription Factors - chemistry
SOX Transcription Factors - genetics
Sox2
Spermatogonia - chemistry
Spermatogonia - metabolism
Spermatogonial stem cell
Testis - chemistry
Transcriptome
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Summary:Because little is known about the function of Sox2 (Sry-related box-2) in teleosts, the objective of this study was to clone and characterize Sox2 complementary DNA (cDNA) from the testis of Indian major carp, Labeo rohita (rohu). The full-length cDNA contained an open reading frame of 936 nucleotides bearing the typical structural features. Phylogenetically, Sox2 of L rohita was most closely related to freshwater counterparts than marine water. The sequence information of cDNA and genomic DNA together revealed that the Sox2 gene is encoded by an uninterrupted exon. Furthermore, comparative mRNA expression profile in various organs including proliferating spermatogonial stem cells (SSCs) suggested about the participatory role of Sox2 during fish male germ cell development and maintenance of stem cells. In support, we have also provided evidence that Sox2 protein is indeed present in rohu SSCs by Western blot analysis. The evolutionarily conserved high-mobility group box domain indicated its possible involvement in common networking pathways for stem cell maintenance and pluripotency between mammals and nonmammals. Our findings could be the first step toward the use of Sox2 as a potential biomarker for proliferating SSCs and understanding the transcriptional regulatory network involved during male germ cell development and maintenance in fish species.
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2015.03.017