Implementation of a manual for working with wobbler mice and criteria for discontinuation of the experiment

Mouse breeding is of importance to a whole range of medical and biological research. There are many known mouse models for motor neuron diseases. However, it must be kept in mind that especially mouse models for amyotrophic lateral sclerosis develop severe symptoms causing intense stress. This artic...

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Bibliographic Details
Published in:Annals of anatomy 2015-07, Vol.200, p.118-124
Main Authors: Ott, Bastian, Dahlke, Carolin, Meller, Karl, Napirei, Markus, Schmitt-John, Thomas, Brand-Saberi, Beate, Theiss, Carsten, Saberi, Darius
Format: Article
Language:English
Subjects:
Amyotrophic lateral sclerosis
Amyotrophic Lateral Sclerosis - genetics
Amyotrophic Lateral Sclerosis - pathology
Amyotrophic Lateral Sclerosis - psychology
Animal care
Animal Husbandry
Animal Welfare
Animals
Breeding
Criteria for discontinuation
Disease Models, Animal
Disease Progression
Genotype
Immunohistochemistry
Manuals as Topic
Mice
Mice, Neurologic Mutants - genetics
Mice, Neurologic Mutants - physiology
Motor Neurons - pathology
Paralysis - pathology
PCR
Phenotype
Stress, Psychological - prevention & control
Tremor - etiology
Tremor - genetics
Weight Loss
Wobbler
Workflow
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Summary:Mouse breeding is of importance to a whole range of medical and biological research. There are many known mouse models for motor neuron diseases. However, it must be kept in mind that especially mouse models for amyotrophic lateral sclerosis develop severe symptoms causing intense stress. This article is designed to summarize conscientious work with the wobbler mouse, a model for the sporadic form of amyotrophic lateral sclerosis. This mouse model is characterized by a degeneration of α-motor-neurons leading to head tremor, loss of body weight and rapidly progressive paralysis. Although this mouse model has been known since 1956, there are no guidelines for breeding wobbler mice. Due to the lack of such guidelines the present study tries to close this gap and implements a manual for further studies. It includes the whole workflow in regard to wobbler mice from breeding and animal care taking, genotyping and phenotype analysis, but also gives some examples for the use of various neuronal tissues for histological investigation. Beside the progress in research a second aim should always be the enhancement of mouse welfare and reduction of stress for the laboratory animals.
ISSN:0940-9602
1618-0402
DOI:10.1016/j.aanat.2015.03.007