Ethylene and pollination decrease transcript abundance of an ethylene receptor gene in Dendrobium petals

• Published in: Journal of plant physiology 2015-03, Vol.176, p.96-100
• Main Authors: Thongkum, Monthathip, Burns, Parichart, Bhunchoth, Anjana, Warin, Nuchnard, Chatchawankanphanich, Orawan, van Doorn, Wouter G.
• Format: Article
• Language: English
• Subjects: 5' Flanking Region - genetics; Abundance; Computer Simulation; Cyclopropanes - pharmacology; Dendrobium; Dendrobium - drug effects; Dendrobium - genetics; Ethylene; Ethylene receptor; Ethylenes - pharmacology; Flower senescence; Flowers; Flowers - drug effects; Flowers - genetics; Gene Expression Profiling; Gene Expression Regulation, Plant - drug effects; Genes; Genes, Plant; Inhibitors; Orchidaceae; Petals; Plant Proteins - genetics; Plant Proteins - metabolism; Pollination - drug effects; Promoter analysis; Receptors; Receptors, Cell Surface - genetics; Receptors, Cell Surface - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sequence Analysis, DNA; Signal Transduction - drug effects; Signal Transduction - genetics; TCP (protocol)
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/j.jplph.2014.12.008

•We studied the expression of a gene encoding an ethylene receptor, called Ethylene Response Sensor 1 (Den-ERS1).•Ethylene and pollination resulted in lower Den-ERS1 abundance.•Inhibition of ethylene production increased abundance.•Promoter binding sites included several transcription factors, some related to ethylene production. We studied the expression of a gene encoding an ethylene receptor, called Ethylene Response Sensor 1 (Den-ERS1), in the petals of Dendrobium orchid flowers. Transcripts accumulated during the young floral bud stage and declined by the time the flowers had been open for several days. Pollination or exposure to exogenous ethylene resulted in earlier flower senescence, an increase in ethylene production and a lower Den-ERS1 transcript abundance. Treatment with 1-methylcyclopropene (1-MCP), an inhibitor of the ethylene receptor, decreased ethylene production and resulted in high transcript abundance. The literature indicates two kinds of ethylene receptor genes with regard to the effects of ethylene. One group shows ethylene-induced down-regulated transcription, while the other has ethylene-induced up-regulation. The present gene is an example of the first group. The 5′ flanking region showed binding sites for Myb and myb-like, homeodomain, MADS domain, NAC, TCP, bHLH and EIN3-like transcription factors. The binding site for the EIN3-like factor might explain the ethylene effect on transcription. A few other transcription factors (RAV1 and NAC) seem also related to ethylene effects.