Colourimetric redox-polyaniline nanoindicator for in situ vesicular trafficking of intracellular transport

Vesicular pH modulates the function of many organelles and plays a pivotal role in cell metabolism processes such as proliferation and apoptosis. Here, we introduce a simple colorimetric redox-polyaniline nanoindicator, which can detect and quantify a broader biogenic pH range with superior sensitiv...

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Bibliographic Details
Published in:Nano research 2015-04, Vol.8 (4), p.1169-1179
Main Authors: Choi, Eun Bi, Choi, Jihye, Bae, Seo Ryung, Kim, Hyun-Ouk, Jang, Eunji, Kang, Byunghoon, Kim, Myeong-Hoon, Kim, Byeongyoon, Suh, Jin-Suck, Lee, Kwangyeol, Huh, Yong-Min, Haam, Seungjoo
Format: Article
Language:English
Subjects:
Aniline
Apoptosis
Atomic/Molecular Structure and Spectra
Biomedicine
Biotechnology
Cell cycle
Chemistry and Materials Science
Condensed Matter Physics
Fluorescence
Materials Science
Nanoparticles
Nanostructure
Nanotechnology
NMR
Nuclear magnetic resonance
Oxidants
Oxidizing agents
pH调节
Polymerization
Protons
Research Article
Sensors
Signal transduction
Silica
Transport
二氧化硅
原位
囊泡运输
比色
氧化还原
聚苯胺
荧光共振能量转移
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Summary:Vesicular pH modulates the function of many organelles and plays a pivotal role in cell metabolism processes such as proliferation and apoptosis. Here, we introduce a simple colorimetric redox-polyaniline nanoindicator, which can detect and quantify a broader biogenic pH range with superior sensitivity compared to pre-established trafficking agents employing one-dimensional turn-on of the fluorescence resonance-energy-transfer （FRET） signal. We fabricated polyaniline- based nanoprobes, which exhibited convertible transition states according to the proton levels, as an in situ indicator of vesicular transport pH. Silica-coated Fe304-MnO heterometal nanoparticles were synthesised and utilised as a metal oxidant to polymerise the aniline monomer. Finally, silica-coated polyaniline nanoparticles with adsorbed cyanine dye fluorophores Cy3 and Cy7 （FPSNIcyB and FPSNIcy7） were fabricated as proton-sensitive nanoindicators. Owing to the selective quenching induced by the local pH variations of vesicular transport, FPSNIcy3 and FPSNIcy7 demonstrated excellent intracellular trafficking and provided sensitive optical indication of minute proton levels.
ISSN:1998-0124
1998-0000
DOI:10.1007/s12274-014-0597-6