Addition of hyaluronic acid improves cellular infiltration and promotes early-stage chondrogenesis in a collagen-based scaffold for cartilage tissue engineering

The response of mesenchymal stem cells (MSCs) to a matrix largely depends on the composition as well as the extrinsic mechanical and morphological properties of the substrate to which they adhere to. Collagen–glycosaminoglycan (CG) scaffolds have been extensively used in a range of tissue engineerin...

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Bibliographic Details
Published in:Journal of the mechanical behavior of biomedical materials 2012-07, Vol.11, p.41-52
Main Authors: Matsiko, Amos, Levingstone, Tanya J., O’Brien, Fergal J., Gleeson, John P.
Format: Article
Language:English
Subjects:
Absorption
Animals
Cartilage
Cartilage - cytology
Cartilage - drug effects
Cartilage - metabolism
Cell Count
Cellular
Chondrogenesis
Chondrogenesis - drug effects
Chondroitin Sulfates - metabolism
Chondroitin sulphate
Collagen
Collagen - chemistry
Collagen - metabolism
Collagen - pharmacology
Collagens
Compressive Strength
Gene Expression Regulation - drug effects
Glycosaminoglycan
Hyaluronic acid
Hyaluronic Acid - metabolism
In vitro testing
Infiltration
Porosity
Rats
Rats, Wistar
Scaffold
Scaffolds
Structure-Activity Relationship
Temperature
Time Factors
Tissue engineering
Tissue Engineering - methods
Tissue Scaffolds - chemistry
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Summary:The response of mesenchymal stem cells (MSCs) to a matrix largely depends on the composition as well as the extrinsic mechanical and morphological properties of the substrate to which they adhere to. Collagen–glycosaminoglycan (CG) scaffolds have been extensively used in a range of tissue engineering applications with great success. This is due in part to the presence of the glycosaminoglycans (GAGs) in complementing the biofunctionality of collagen. In this context, the overall goal of this study was to investigate the effect of two GAG types: chondroitin sulphate (CS) and hyaluronic acid (HyA) on the mechanical and morphological characteristics of collagen-based scaffolds and subsequently on the differentiation of rat MSCs in vitro. Morphological characterisation revealed that the incorporation of HyA resulted in a significant reduction in scaffold mean pore size (93.9μm) relative to collagen–CS (CCS) scaffolds (136.2μm). In addition, the collagen–HyA (CHyA) scaffolds exhibited greater levels of MSC infiltration in comparison to the CCS scaffolds. Moreover, these CHyA scaffolds showed significant acceleration of early stage gene expression of SOX-9 (approximately 60-fold higher, p
ISSN:1751-6161
1878-0180
DOI:10.1016/j.jmbbm.2011.11.012