Ethanol fermentation using a glucose negative mutant of Zymomonas mobilis

The fermentation of an equimolar mixture of glucose and fructose into ethanol and sorbitol by a glucose negative mutant of Zymomonas mobilis was monitored. The results were analyzed using a recently described method based on polynomial fitting and calculation of "intantaneous" and "ov...

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Bibliographic Details
Published in:Biotechnology techniques 1995-05, Vol.9 (5), p.315-320
Main Authors: AÏT-ABDELKADER, N, BARATTI, J. C
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Zymomonas mobilis
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Summary:The fermentation of an equimolar mixture of glucose and fructose into ethanol and sorbitol by a glucose negative mutant of Zymomonas mobilis was monitored. The results were analyzed using a recently described method based on polynomial fitting and calculation of "intantaneous" and "overall" parameters. These parameters described well the physiology of this mixed-substrate mixed-product fermentation. Growth of the mutant was greatly inhibited on this medium. Fructose was quantitatively converted into sorbitol while glucose was oxidized into gluconic acid. This latter product was utilized as substrate for cell growth and ethanol production.
ISSN:0951-208X
1573-6784
DOI:10.1007/BF00638862