Gastroprotective potential of Buddleja scordioides Kunth Scrophulariaceae infusions; effects into the modulation of antioxidant enzymes and inflammation markers in an in vivo model

• Published in: Journal of ethnopharmacology 2015-07, Vol.169, p.280-286
• Main Authors: Díaz-Rivas, J.O., Herrera-Carrera, E., Gallegos-Infante, J.A., Rocha-Guzmán, N.E., González-Laredo, R.F., Moreno-Jiménez, M.R., Ramos-Gómez, M., Reynoso-Camacho, R., Larrosa-Pérez, M., Gallegos-Corona, M.A.
• Format: Article
• Language: English
• Subjects: Animals; Anti-Ulcer Agents - pharmacology; Antioxidants - metabolism; Biomarkers - metabolism; Buddleja - chemistry; Buddleja scordioides; Down-Regulation - drug effects; Gastric damage; Indomethacin; Indomethacin - adverse effects; Inflammation; Inflammation Mediators - metabolism; Male; Nitrites - metabolism; NSAIDs; Plant Extracts - pharmacology; Plant Leaves - chemistry; Rats; Stomach - drug effects; Stomach - metabolism; Stomach - pathology; Stomach Ulcer - chemically induced; Stomach Ulcer - drug therapy; Stomach Ulcer - prevention & control; Up-Regulation - drug effects
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2015.04.024

A common plant used to treat several gastric disorders is Buddleja scordioides Kunth, commonly known as salvilla. To detect inflammatory markers, in order to evaluate the gastroprotective potential of salvilla infusions, as this could have beneficial impact on the population exposed to gastric ulcers and colitis. The present work attempted infusions were prepared with B. scordioides (1% w/w) lyophilized and stored. Total phenolic content and GC–MS analysis were performed. Wistar rats were divided into five groups (n=8), a negative vehicle control, an indomethacin group, and three experimental groups, named preventive, curative, and suppressive. All rats were sacrificed under deep ether anesthesia (6h) after the last oral administration of indomethacin/infusion. The rat stomachs were promptly excised, weighed, and chilled in ice-cold and 0.9% NaCl. Histological analysis, nitrites quantification and immunodetection assays were done. B. scordioides infusions markedly reduced the visible hemorrhagic lesions induced by indomethacin in rat stomachs, also showed down-regulation of COX2, IL-8 and TNFα and up-regulation of COX-1 with a moderate down-regulation of NFkB and lower amount of nitrites. However, this behavior was dependent on the treatment, showing most down-regulation of COX-2, TNFα and IL-8 in the curative treatment; more down-regulation of NF-kB in the preventive treatment; and more up-regulation of COX-1 for the suppressor and preventive treatments. The anti-inflammatory potential of B. scordioides infusions could be related with the presence of polyphenols as quercetin in the infusion and how this one is consumed. [Display omitted]