Identification of Naemacyclus minor hyphae within needle tissues of Pinus sylvestris to immunoelectron microscopy

Cultural investigations revealed that Naemacyclus minor, Lophodermium seditiosum and Cenangium ferruginosum were the most frequent colonizers of asymptomatic and symptomatic Pinus sylvestris needles. Since ultrastructural observations showed that morphological features were not suitable to different...

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Bibliographic Details
Published in:Archives of microbiology 1993-10, Vol.160 (4), p.265-272
Main Authors: Franz, F, Grotjahn, R, Acker, G
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
conifer needles
Cyclaneusma minus
Fundamental and applied biological sciences. Psychology
hyphae
immunocytochemistry
immunogold technique
immunohistochemistry
immunologic techniques
Microbiology
Mycology
Pathogenicity, host-agent relations, miscellaneous strains, epidemiology
Pinus sylvestris
stomata
transmission electron microscopy
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Summary:Cultural investigations revealed that Naemacyclus minor, Lophodermium seditiosum and Cenangium ferruginosum were the most frequent colonizers of asymptomatic and symptomatic Pinus sylvestris needles. Since ultrastructural observations showed that morphological features were not suitable to differentiate hyphae of N. minor from hyphae of other isolates, the on-section immunogold labelling technique was applied in combination with an anti-N. minor specific immunoserum. The specificity of this serum was tested against culture hyphae of all isolates. Anti-N. minor specific immunoserum was then used to identify N. minor hyphae in thin sections of green P. sylvestris needles. The infection loci identified were restricted to small tissue areas located in the vicinity of stomata. In the hypodermis, hyphae and endocell-containing hyphae were located within the lumina of host cells but outside the protoplast. The growth of hyphae from cell to cell occurred through pits. The hyphae spread into the mesophyll intercellularly and continued with the intracellular colonization of moribund and dead mesophyll cells in a later stage of infection. The observed host-parasite interactions at cellular and ultrastructural level are discussed in connection with the still controversial interpretation of the pathogenicity of N. minor.
ISSN:0302-8933
1432-072X
DOI:10.1007/BF00292075