Morphological and genetic characterization of group I Clostridium botulinum type B strain 111 and the transcriptional regulator spoIIID gene knockout mutant in sporulation

• Published in: Anaerobe 2015-06, Vol.33, p.55-63
• Main Authors: Hosomi, Koji, Kuwana, Ritsuko, Takamatsu, Hiromu, Kohda, Tomoko, Kozaki, Shunji, Mukamoto, Masafumi
• Format: Article
• Language: English
• Subjects: Bacterial Proteins - genetics; Clostridium botulinum; Clostridium botulinum type B - classification; Clostridium botulinum type B - physiology; Gene Expression Regulation, Bacterial; Gene Knockout Techniques; Gene Order; Gene Targeting; Models, Biological; Mutation; Phenotype; Spores, Bacterial; Sporulation; Transcription Factors - genetics; Transcription, Genetic; Transcriptional regulator spoIIID
• ISSN: 1075-9964; 1095-8274
• DOI: 10.1016/j.anaerobe.2015.01.012

Clostridium botulinum is a heat-resistant spore-forming bacterium that causes the serious paralytic illness botulism. Heat-resistant spores may cause food sanitation hazards and sporulation plays a central role in the survival of C. botulinum. We observed morphological changes and investigated the role of the transcriptional regulator SpoIIID in the sporulation of C. botulinum type B strain 111 in order to elucidate the molecular mechanism in C. botulinum. C. botulinum type B formed heat-resistant spores through successive morphological changes corresponding to those of Bacillus subtilis, a spore-forming model organism. An analysis of the spoIIID gene knockout mutant revealed that the transcriptional regulator SpoIIID contributed to heat-resistant spore formation by C. botulinum type B and activated the transcription of the sigK gene later during sporulation. Transcription of the spoIIID gene, which differed from that in B. subtilis and Clostridium difficile, was observed in the sigE gene knockout mutant of C. botulinum type B. An analysis of the sigF gene knockout mutant showed that the sporulation-specific sigma factor SigF was essential for transcription of the spoIIID gene in C. botulinum type B. These results suggest that the regulation of sporulation in C. botulinum is not similar to that in B. subtilis and other clostridia. •We observed sporulation of Clostridium botulinum type B strain 111.•We investigated the role of the transcriptional regulator SpoIIID.•SpoIIID contributed to heat-resistant spore formation by C. botulinum type B.•SpoIIID activated the transcription of the sigK gene later during sporulation.•Sigma factor SigF was essential for transcription of the spoIIID gene.