Effect of sodium accumulation on heterotrophic growth and polyhydroxybutyrate (PHB) production by Cupriavidus necator

•A mechanistic model was developed to evaluate the effect of Na+ on PHB production.•Model calibration and validation was performed with batch and fed-batch data sets.•Both the steady state and dynamic behavior was effected by Na+.•Non-linear inhibition effect of Na+ on growth and PHB content.•Na+ sh...

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Bibliographic Details
Published in:Bioresource technology 2015-09, Vol.191, p.213-218
Main Authors: Mozumder, Md. Salatul Islam, Garcia-Gonzalez, Linsey, De Wever, Heleen, Volcke, Eveline I.P.
Format: Article
Language:English
Subjects:
biomass
Biomass growth
Calibration
culture flasks
Cupriavidus necator
Cupriavidus necator - metabolism
Hydroxybutyrates - metabolism
Mathematical modeling
mathematical models
Models, Biological
Na+ inhibition
polyhydroxybutyrate
Polyhydroxybutyrate (PHB) production
Simulation
sodium
Sodium - metabolism
technology
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Summary:•A mechanistic model was developed to evaluate the effect of Na+ on PHB production.•Model calibration and validation was performed with batch and fed-batch data sets.•Both the steady state and dynamic behavior was effected by Na+.•Non-linear inhibition effect of Na+ on growth and PHB content.•Na+ shows linear inhibition effect on PHB production rate. This study evaluates the effect of sodium (Na+) concentration on the growth and PHB production by Cupriavidus necator. Both biomass growth and PHB production were inhibited by Na+: biomass growth became zero at 8.9g/L Na+ concentration while PHB production was completely stopped at 10.5g/L Na+. A mathematical model for pure culture heterotrophic PHB production was set up to describe the Na+ inhibition effect. The parameters related to Na+ inhibition were estimated based on shake flask experiments. The accumulated Na+ showed non-linear inhibition effect on biomass growth but linear inhibition effect on PHB production kinetics. Fed-batch experiments revealed that a high accumulation of Na+ due to a prolonged growth phase, using NaOH for pH control, decreased the subsequent PHB production. The model was validated based on independent experimental data sets, showing a good agreement between experimental data and simulation results.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2015.04.110