A novel in vitro toxicological approach to dentify chemicals with a prostate-mediated effect on male reproduction

Prostote, an overlooked target in in vitro alternative methods, is critical for male fertility. Within the EU project ReProTect, the LNCaP cell line was used as a model system to screen chemicals affecting prostate by a tiered approach integrating two toxicological endpoints: cell viability and PSA...

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Bibliographic Details
Published in:Journal of Biological Research 2011-01, Vol.84 (1), p.36-36
Main Authors: Lorenzetti, S, Narciso, L, Marcoccia, D, Altieri, I
Format: Article
Language:English
Online Access:Get full text
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Summary:Prostote, an overlooked target in in vitro alternative methods, is critical for male fertility. Within the EU project ReProTect, the LNCaP cell line was used as a model system to screen chemicals affecting prostate by a tiered approach integrating two toxicological endpoints: cell viability and PSA secretion. A ReProTect training set of(anti) androgenic chemicals and a ReProTect fe set of blinded chemicals affecting reprodut tissues were used. Androgens, and ui glufosinate ammonium, markedly if whereas anti-androgens also increase at a much lower magnitude than androgens. Our tiered approach properly duscriminated adrogenic compounds as well as yielded no false posriives, as based on available toxnicilogical evidences. The PSA secretion assay is directly linked to the prostate physiological function and it may integrate the information provided by mechanistic-based assays (i.e., AR binding and gene expression).
ISSN:1826-8838
2284-0230