The Effect of Charge at the Surface of Silver Nanoparticles on Antimicrobial Activity against Gram-Positive and Gram-Negative Bacteria: A Preliminary Study

The bactericidal efficiency of various positively and negatively charged silver nanoparticles has been extensively evaluated in literature, but there is no report on efficacy of neutrally charged silver nanoparticles. The goal of this study is to evaluate the role of electrical charge at the surface...

Full description

Saved in:
Bibliographic Details
Published in:Journal of nanomaterials 2015-01, Vol.2015 (2015), p.1-8
Main Authors: Sharghi, Hashem, Dorostkar, Samira, Hemmateenejad, Bahram, Gholami, Ahmad, Ghahramani, Yasamin, Abbaszadegan, Abbas, Nabavizadeh, Mohammadreza
Format: Article
Language:English
Subjects:
Antiinfectives and antibacterials
Antimicrobial agents
Bacteria
Bacteriology
Biological activity
Charged particles
Charging
Colleges & universities
Escherichia coli
Gram-negative bacteria
Gram-positive bacteria
Microorganisms
Nanomaterials
Nanoparticles
Proteus vulgaris
Silver
Staphylococcus aureus
Streptococcus
Streptococcus mutans
Streptococcus pyogenes
Surface chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The bactericidal efficiency of various positively and negatively charged silver nanoparticles has been extensively evaluated in literature, but there is no report on efficacy of neutrally charged silver nanoparticles. The goal of this study is to evaluate the role of electrical charge at the surface of silver nanoparticles on antibacterial activity against a panel of microorganisms. Three different silver nanoparticles were synthesized by different methods, providing three different electrical surface charges (positive, neutral, and negative). The antibacterial activity of these nanoparticles was tested against gram-positive (i.e., Staphylococcus aureus, Streptococcus mutans, and Streptococcus pyogenes) and gram-negative (i.e., Escherichia coli and Proteus vulgaris) bacteria. Well diffusion and micro-dilution tests were used to evaluate the bactericidal activity of the nanoparticles. According to the obtained results, the positively-charged silver nanoparticles showed the highest bactericidal activity against all microorganisms tested. The negatively charged silver nanoparticles had the least and the neutral nanoparticles had intermediate antibacterial activity. The most resistant bacteria were Proteus vulgaris. We found that the surface charge of the silver nanoparticles was a significant factor affecting bactericidal activity on these surfaces. Although the positively charged nanoparticles showed the highest level of effectiveness against the organisms tested, the neutrally charged particles were also potent against most bacterial species.
ISSN:1687-4110
1687-4129
DOI:10.1155/2015/720654