Ultrastructure of head organs (anterior adhesive apparatus) and posterior secretory systems of Caballeria liewi Lim, 1995 (Monogenea, Ancyrocephalidae)

• Published in: Parasitology research (1987) 2014-11, Vol.113 (11), p.3935-3946
• Main Authors: Wong, Wey-Lim, Brennan, Gerard P, Halton, David W, Maule, Aaron G, Lim, Lee-Hong Susan
• Format: Article
• Language: English
• Subjects: Ancyrocephalidae; Animals; Biomedical and Life Sciences; Biomedicine; Bodily Secretions; endoplasmic reticulum; exocytosis; exudation; Golgi apparatus; head; Head - anatomy & histology; Immunology; locomotion; Medical Microbiology; Microbiology; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; microvilli; Monogenea; Original Paper; Pharynx; secretion; Secretory Pathway; Trematoda - ultrastructure; ultrastructure
• ISSN: 0932-0113; 1432-1955
• DOI: 10.1007/s00436-014-4057-8

Caballeria liewi Lim, 1995, uses adhesive secretions from the head organs and posterior secretory systems to assist in locomotion and attachment. Ultrastructural investigations show that the head organs of C. liewi consist of three pairs of antero-lateral pit-like openings bearing microvilli and ducts leading from two types of uninucleated gland cells (located lateral to the pharynx), one type producing rod-like (S1) bodies with an electron-dense matrix containing less electron-dense vesicles and the second type producing oval (S2) bodies with a homogeneous electron-dense matrix. Interlinking band-like structures are observed between S1 bodies and between S2 bodies. S1 body is synthesised in the granular endoplasmic reticulum, transported to a Golgi complex to be packaged into vesicles and routed into ducts for exudation. The synthesis of the S2 body is unresolved. Haptoral secretions manifested externally as net-like structures are derived from dual electron-dense (DED) secretory body produced in the peduncular gland cells. The DED body consists of a less electron-dense oval core in a homogeneous electron-dense matrix. On exocytosis into the pyriform haptoral reservoir, DED bodies are transformed into a secretion with two types of inclusions (less electron-dense oval and electron-dense spherical inclusions) in an electron-dense matrix. The secretions are further transformed (as small, oval, electron-dense bodies) when transported to the superficial anchor grooves, and on exudation into the gill tissues, the secretions become an electron-dense matrix. Secretory bodies associated with uniciliated structures, anchor sleeves and marginal hooks are also observed.