Different effects of lobeline on neuronal and muscle nicotinic receptors

Lobeline is a plant alkaloid known to interact with cholinergic system. The effect of lobeline on neuronal α3β4 receptors expressed in COS cells and muscle embryonic αβγδ receptors naturally expressed in TE671 cells was studied using a patch-clamp technique. Our results show that lobeline inhibited...

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Bibliographic Details
Published in:European journal of pharmacology 2014-09, Vol.738, p.352-359
Main Authors: Kaniakova, Martina, Skrenkova, Kristyna, Adamek, Svatopluk, Vyskocil, Frantisek, Krusek, Jan
Format: Article
Language:English
Subjects:
Animals
Cercopithecus aethiops
COS Cells
Humans
Lobeline - pharmacology
Muscles - drug effects
Muscles - metabolism
Neurons - drug effects
Neurons - metabolism
Nicotinic Agonists - pharmacology
Organ Specificity
Rats
Receptors, Nicotinic - metabolism
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Summary:Lobeline is a plant alkaloid known to interact with cholinergic system. The effect of lobeline on neuronal α3β4 receptors expressed in COS cells and muscle embryonic αβγδ receptors naturally expressed in TE671 cells was studied using a patch-clamp technique. Our results show that lobeline inhibited responses to acetylcholine in human embryonic muscle nicotinic receptor in a pseudo-noncompetitive manner. The responses of rat neuronal α3β4 receptors to a low concentration of acetylcholine were potentiated by a mixed occupation mechanism that corresponds to "competitive potentiation". This potentiation turned into voltage-dependent inhibition for α3β4 receptors was activated by a high concentration of acetylcholine.
ISSN:0014-2999
1879-0712
DOI:10.1016/j.ejphar.2014.05.057