Transformation of Salmonella typhimurium TA1538 by optimized electroporation

Standard electroporation of Salmonella typhimurium TA1538 by the plasmid pAMH70 yielded 1.3x10 super(2) transformants/ mu g of plasmid DNA. Three parameters: resistance (U sub(1)), voltage (U sub(2)) and dose of plasmid DNA (U sub(3)) were optimized with two Doehlert designs. Transformation was incr...

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Bibliographic Details
Published in:Biotechnology techniques 1993-04, Vol.7 (4), p.255-260
Main Authors: GENTIL, M, AZAS, N, DE MEO, M, LAGET, M, DUMENIL, G
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Methods. Procedures. Technologies
Miscellaneous
Salmonella typhimurium
Synthetic digonucleotides and genes. Sequencing
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Summary:Standard electroporation of Salmonella typhimurium TA1538 by the plasmid pAMH70 yielded 1.3x10 super(2) transformants/ mu g of plasmid DNA. Three parameters: resistance (U sub(1)), voltage (U sub(2)) and dose of plasmid DNA (U sub(3)) were optimized with two Doehlert designs. Transformation was increased 75 fold. Optimal values for U sub(1), U sub(2) and U sub(3) were 761.0 Omega , 1.6 kV and 96.1 ng respectively. The most important parameters were U sub(2) and the couple U sub(1)/U sub(2).
ISSN:0951-208X
1573-6784
DOI:10.1007/BF00150894