Oleanane-type saponins from Glochidion glomerulatum and their cytotoxic activities

• Published in: Phytochemistry (Oxford) 2015-08, Vol.116, p.213-220
• Main Authors: Thu, Vu Kim, Van Thang, Nguyen, Nhiem, Nguyen Xuan, Tai, Bui Huu, Nam, Nguyen Hoai, Kiem, Phan Van, Minh, Chau Van, Anh, Hoang Le Tuan, Kim, Nanyoung, Park, Seonju, Kim, Seung Hyun
• Format: Article
• Language: English
• Subjects: Antineoplastic Agents, Phytogenic - chemistry; Antineoplastic Agents, Phytogenic - isolation & purification; Antineoplastic Agents, Phytogenic - pharmacology; Cytotoxic activity; Drug Screening Assays, Antitumor; Euphorbiaceae; Euphorbiaceae - chemistry; Female; Glochidion glomerulatum; HT29 Cells; Humans; Nuclear Magnetic Resonance, Biomolecular; Oleanane-type saponins; Oleanolic Acid - analogs & derivatives; Oleanolic Acid - chemistry; Oleanolic Acid - isolation & purification; Oleanolic Acid - pharmacology; Plant Leaves - chemistry; Saponins - chemistry; Saponins - isolation & purification; Saponins - pharmacology; Vietnam
• ISSN: 0031-9422; 1873-3700
• DOI: 10.1016/j.phytochem.2015.05.001

Eight oleanane-type saponins were isolated from leaves of Glochidion glomerulatum. Compounds 3 and 5 which contain a benzoyloxy group at C-22 showed significant cytotoxic activities against the A-549, HT-29, and OVCAR cancer cell lines with IC50 values ranging from 5.9 to 9.8μM. [Display omitted] •Eight oleanane-type saponins, glomerulosides A–H were isolated from leaves of Glochidion glomerulatum.•The cytotoxic activities of all compounds were evaluated against A-549, HT-29, and OVCAR, and MCF-7 cancer cell lines.•Glomerulosides A–C and E showed significant cytotoxic activities. Eight oleanane-type saponins, glomerulosides A–H, were isolated from leaves of Glochidion glomerulatum. All isolated compounds were evaluated for cytotoxic activity on four human cancer cell lines, A-549, HT-29, OVCAR, and MCF-7. Glomerulosides C and E, which contain a benzoyloxy group at C-22, showed significant cytotoxic activities against the A-549, HT-29, and OVCAR cancer cell lines with IC50 values ranging from 5.9 to 9.8μM. Glomeruloside A showed cytotoxicity on HT-29 and OVCAR cell lines with IC50 values of 7.3 and 6.6μM, respectively. Moreover, glomeruloside B exhibited significant activity on A-549 and HT-29 cancer cell lines with IC50 values of 9.7 and 7.5μM. In contrast, glomerulosides F–H, lacking a benzoyloxy group, showed only moderate cytotoxic activity.