Studies on the Determination of Biogenic Amines in Foods. 1. Development of a TLC Method for the Determination of Eight Biogenic Amines in Fish

Ingestion of foods that contain high levels of histamine causes clinical symptoms, namely histamine poisoning. During the course of this investigation eight biogenic amines in fish samples, including histamine, putrescine, cadaverine, tyramine, tryptamine, spermine, spermidine, and beta-phenylethyla...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1995-01, Vol.43 (1), p.134-139
Main Authors: Naguib, Khayria, Ayesh, A. M, Shalaby, A. R
Format: Article
Language:English
Subjects:
ALIMENTOS
AMINAS
AMINAS BIOGENICAS
AMINE
AMINE BIOGENE
ANALISIS CUANTITATIVO
ANALYSE QUANTITATIVE
Brackish
CHLORURE
CHROMATOGRAPHIE EN COUCHE MINCE
CLORUROS
CROMATOGRAFIA DE CAPA FINA
ENVENENAMIENTO
ESPERMIDINA
ESPERMINA
Freshwater
HISTAMINA
HISTAMINE
INTOXICATION
Marine
PRODUIT ALIMENTAIRE
PUTRESCINA
PUTRESCINE
SARDINA
SARDINE
SEPARACION
SEPARATION
SPERMIDINE
SPERMINE
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Summary:Ingestion of foods that contain high levels of histamine causes clinical symptoms, namely histamine poisoning. During the course of this investigation eight biogenic amines in fish samples, including histamine, putrescine, cadaverine, tyramine, tryptamine, spermine, spermidine, and beta-phenylethylamine, were separated and quantitated by TLC technique. Many solvent systems were evaluated, with the best separation in one dimension when benzene/triethylamine (5:1) was used as a developing system, which enabled separation of histamine in addition to spermine, tyramine, and beta-phenylethylamine. However, the other four biogenic amines were not separated using this developing system. The only way to separate the eight tested biogenic amines was by using a two-dimensional TLC procedure. The running system in the first direction was benzene/triethylamine/acetone (10:2:1), and the solvent system in the second direction was benzene/triethylamine (5:1). This method provides a simple and inexpensive means to separate and detect biogenic amines in fish samples
ISSN:0021-8561
1520-5118
DOI:10.1021/jf00049a025