Azocasein assay for alkaline protease in complex fermentation broth

An azocasein assay has been developed for determination of alkaline protease in fermentation broth from a complex substrate containing ca. 50 g/l protein which to a high degree interferes with azocasein. Methods described in the literature have been found inaccurate as results deviate ca. 50% from t...

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Bibliographic Details
Published in:Biotechnology techniques 1995-08, Vol.9 (8), p.573-576
Main Authors: IVERSEN, S. L, JØRGENSEN, M. H
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Others
Various methods and equipments
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Summary:An azocasein assay has been developed for determination of alkaline protease in fermentation broth from a complex substrate containing ca. 50 g/l protein which to a high degree interferes with azocasein. Methods described in the literature have been found inaccurate as results deviate ca. 50% from true activity, so one of the methods is modified in order to eliminate interference. Proportionality between the relative azocasein concentration and the deviation from true activity is found. An azocasein concentration of 350 mg azocasein per ml sample gives satisfactory results with an accuracy of plus or minus 5%. Application of a standard addition method also improves the accuracy, but is laborious and less precise.
ISSN:0951-208X
1573-6784
DOI:10.1007/BF00152446