Bioactivity and mechanism of action of Lophozozymus pictor toxin

The bioactivity of Lophozozymus pictor toxin (LPTX) and the possible mechanism of action of the purified toxin are described. LPTX is found to possess palytoxin-like bioactivities. Besides exhibiting cytotoxic and haemolytic properties, LPTX causes the release of K + from erythrocytes and inhibits 2...

Full description

Saved in:
Bibliographic Details
Published in:Toxicon (Oxford) 1995-07, Vol.33 (7), p.901-908
Main Authors: Lau, C.O., Tan, C.H., Li, Q.T., Ng, F.H., Yuen, R., Khoo, H.E.
Format: Article
Language:English
Subjects:
Animal poisons toxicology. Antivenoms
Animals
Biological and medical sciences
Brachyura - chemistry
HeLa Cells
Humans
Lophozozymus pictor
Marine
Marine Toxins - antagonists & inhibitors
Marine Toxins - chemistry
Marine Toxins - pharmacology
Medical sciences
Mitochondria, Liver - drug effects
Ouabain - pharmacology
Rats
Rats, Wistar
Sodium-Potassium-Exchanging ATPase - drug effects
Sphingomyelins - pharmacology
Toxicology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c417t-f283fd9ba400cabe8342a9ca4e0029c93675ae7d10f1d52258677ee4e8d9bcd43
cites cdi_FETCH-LOGICAL-c417t-f283fd9ba400cabe8342a9ca4e0029c93675ae7d10f1d52258677ee4e8d9bcd43
container_end_page 908
container_issue 7
container_start_page 901
container_title Toxicon (Oxford)
container_volume 33
creator Lau, C.O.
Tan, C.H.
Li, Q.T.
Ng, F.H.
Yuen, R.
Khoo, H.E.
description The bioactivity of Lophozozymus pictor toxin (LPTX) and the possible mechanism of action of the purified toxin are described. LPTX is found to possess palytoxin-like bioactivities. Besides exhibiting cytotoxic and haemolytic properties, LPTX causes the release of K + from erythrocytes and inhibits 2-[ 14C]deoxy-D-glucose uptake into HeLa cells. Although LPTX acts on HeLa cell and erythrocyte membranes, it does not interact with mitochondrial or liposomal membranes containing different phospholipid compositions. Ouabain, but not sphingomyelin, is able to prevent the toxic effects of LPTX. This antagonistic effect of ouabain on LPTX suggests that the toxin might mediate its toxic effects via the membrane Na + K + - ATPase but not through interaction with membrane lipids.
doi_str_mv 10.1016/0041-0101(95)00028-K
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_16883086</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>004101019500028K</els_id><sourcerecordid>16883086</sourcerecordid><originalsourceid>FETCH-LOGICAL-c417t-f283fd9ba400cabe8342a9ca4e0029c93675ae7d10f1d52258677ee4e8d9bcd43</originalsourceid><addsrcrecordid>eNp9kE1LAzEQhoMotVb_gcIeRPSwmmyS3eQiavGLFrzoOaTZWYx0NzXZLba_3qwtPXqagfd5h-FB6JTga4JJfoMxIymO66XkVxjjTKSTPTQkopApJRzvo-EOOURHIXxFiAqZD9BAcCEywobo7sE6bVq7tO0q0U2Z1GA-dWNDnbgq6RPX9NvULT7d2q1XdReShTWt80nrfmxzjA4qPQ9wsp0j9PH0-D5-Sadvz6_j-2lqGCnatMoErUo50wxjo2cgKMu0NJpBfFwaSfOCayhKgitS8izjIi8KAAYilkzJ6AhdbO4uvPvuILSqtsHAfK4bcF1QJBeCYpFHkG1A410IHiq18LbWfqUIVr041VtRvRUlufoTpyaxdra9381qKHelramYn29zHYyeV143xoYdRrlknPKI3W4wiC6WFrwKxkJjoLQeTKtKZ___4xejRIlq</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16883086</pqid></control><display><type>article</type><title>Bioactivity and mechanism of action of Lophozozymus pictor toxin</title><source>ScienceDirect Freedom Collection</source><creator>Lau, C.O. ; Tan, C.H. ; Li, Q.T. ; Ng, F.H. ; Yuen, R. ; Khoo, H.E.</creator><creatorcontrib>Lau, C.O. ; Tan, C.H. ; Li, Q.T. ; Ng, F.H. ; Yuen, R. ; Khoo, H.E.</creatorcontrib><description>The bioactivity of Lophozozymus pictor toxin (LPTX) and the possible mechanism of action of the purified toxin are described. LPTX is found to possess palytoxin-like bioactivities. Besides exhibiting cytotoxic and haemolytic properties, LPTX causes the release of K + from erythrocytes and inhibits 2-[ 14C]deoxy-D-glucose uptake into HeLa cells. Although LPTX acts on HeLa cell and erythrocyte membranes, it does not interact with mitochondrial or liposomal membranes containing different phospholipid compositions. Ouabain, but not sphingomyelin, is able to prevent the toxic effects of LPTX. This antagonistic effect of ouabain on LPTX suggests that the toxin might mediate its toxic effects via the membrane Na + K + - ATPase but not through interaction with membrane lipids.</description><identifier>ISSN: 0041-0101</identifier><identifier>EISSN: 1879-3150</identifier><identifier>DOI: 10.1016/0041-0101(95)00028-K</identifier><identifier>PMID: 8588214</identifier><identifier>CODEN: TOXIA6</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animal poisons toxicology. Antivenoms ; Animals ; Biological and medical sciences ; Brachyura - chemistry ; HeLa Cells ; Humans ; Lophozozymus pictor ; Marine ; Marine Toxins - antagonists &amp; inhibitors ; Marine Toxins - chemistry ; Marine Toxins - pharmacology ; Medical sciences ; Mitochondria, Liver - drug effects ; Ouabain - pharmacology ; Rats ; Rats, Wistar ; Sodium-Potassium-Exchanging ATPase - drug effects ; Sphingomyelins - pharmacology ; Toxicology</subject><ispartof>Toxicon (Oxford), 1995-07, Vol.33 (7), p.901-908</ispartof><rights>1995</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-f283fd9ba400cabe8342a9ca4e0029c93675ae7d10f1d52258677ee4e8d9bcd43</citedby><cites>FETCH-LOGICAL-c417t-f283fd9ba400cabe8342a9ca4e0029c93675ae7d10f1d52258677ee4e8d9bcd43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=3594535$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8588214$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lau, C.O.</creatorcontrib><creatorcontrib>Tan, C.H.</creatorcontrib><creatorcontrib>Li, Q.T.</creatorcontrib><creatorcontrib>Ng, F.H.</creatorcontrib><creatorcontrib>Yuen, R.</creatorcontrib><creatorcontrib>Khoo, H.E.</creatorcontrib><title>Bioactivity and mechanism of action of Lophozozymus pictor toxin</title><title>Toxicon (Oxford)</title><addtitle>Toxicon</addtitle><description>The bioactivity of Lophozozymus pictor toxin (LPTX) and the possible mechanism of action of the purified toxin are described. LPTX is found to possess palytoxin-like bioactivities. Besides exhibiting cytotoxic and haemolytic properties, LPTX causes the release of K + from erythrocytes and inhibits 2-[ 14C]deoxy-D-glucose uptake into HeLa cells. Although LPTX acts on HeLa cell and erythrocyte membranes, it does not interact with mitochondrial or liposomal membranes containing different phospholipid compositions. Ouabain, but not sphingomyelin, is able to prevent the toxic effects of LPTX. This antagonistic effect of ouabain on LPTX suggests that the toxin might mediate its toxic effects via the membrane Na + K + - ATPase but not through interaction with membrane lipids.</description><subject>Animal poisons toxicology. Antivenoms</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Brachyura - chemistry</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Lophozozymus pictor</subject><subject>Marine</subject><subject>Marine Toxins - antagonists &amp; inhibitors</subject><subject>Marine Toxins - chemistry</subject><subject>Marine Toxins - pharmacology</subject><subject>Medical sciences</subject><subject>Mitochondria, Liver - drug effects</subject><subject>Ouabain - pharmacology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Sodium-Potassium-Exchanging ATPase - drug effects</subject><subject>Sphingomyelins - pharmacology</subject><subject>Toxicology</subject><issn>0041-0101</issn><issn>1879-3150</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LAzEQhoMotVb_gcIeRPSwmmyS3eQiavGLFrzoOaTZWYx0NzXZLba_3qwtPXqagfd5h-FB6JTga4JJfoMxIymO66XkVxjjTKSTPTQkopApJRzvo-EOOURHIXxFiAqZD9BAcCEywobo7sE6bVq7tO0q0U2Z1GA-dWNDnbgq6RPX9NvULT7d2q1XdReShTWt80nrfmxzjA4qPQ9wsp0j9PH0-D5-Sadvz6_j-2lqGCnatMoErUo50wxjo2cgKMu0NJpBfFwaSfOCayhKgitS8izjIi8KAAYilkzJ6AhdbO4uvPvuILSqtsHAfK4bcF1QJBeCYpFHkG1A410IHiq18LbWfqUIVr041VtRvRUlufoTpyaxdra9381qKHelramYn29zHYyeV143xoYdRrlknPKI3W4wiC6WFrwKxkJjoLQeTKtKZ___4xejRIlq</recordid><startdate>19950701</startdate><enddate>19950701</enddate><creator>Lau, C.O.</creator><creator>Tan, C.H.</creator><creator>Li, Q.T.</creator><creator>Ng, F.H.</creator><creator>Yuen, R.</creator><creator>Khoo, H.E.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H97</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope></search><sort><creationdate>19950701</creationdate><title>Bioactivity and mechanism of action of Lophozozymus pictor toxin</title><author>Lau, C.O. ; Tan, C.H. ; Li, Q.T. ; Ng, F.H. ; Yuen, R. ; Khoo, H.E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-f283fd9ba400cabe8342a9ca4e0029c93675ae7d10f1d52258677ee4e8d9bcd43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animal poisons toxicology. Antivenoms</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Brachyura - chemistry</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Lophozozymus pictor</topic><topic>Marine</topic><topic>Marine Toxins - antagonists &amp; inhibitors</topic><topic>Marine Toxins - chemistry</topic><topic>Marine Toxins - pharmacology</topic><topic>Medical sciences</topic><topic>Mitochondria, Liver - drug effects</topic><topic>Ouabain - pharmacology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Sodium-Potassium-Exchanging ATPase - drug effects</topic><topic>Sphingomyelins - pharmacology</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lau, C.O.</creatorcontrib><creatorcontrib>Tan, C.H.</creatorcontrib><creatorcontrib>Li, Q.T.</creatorcontrib><creatorcontrib>Ng, F.H.</creatorcontrib><creatorcontrib>Yuen, R.</creatorcontrib><creatorcontrib>Khoo, H.E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) 1: Biological Sciences &amp; Living Resources</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) 3: Aquatic Pollution &amp; Environmental Quality</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Toxicon (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lau, C.O.</au><au>Tan, C.H.</au><au>Li, Q.T.</au><au>Ng, F.H.</au><au>Yuen, R.</au><au>Khoo, H.E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bioactivity and mechanism of action of Lophozozymus pictor toxin</atitle><jtitle>Toxicon (Oxford)</jtitle><addtitle>Toxicon</addtitle><date>1995-07-01</date><risdate>1995</risdate><volume>33</volume><issue>7</issue><spage>901</spage><epage>908</epage><pages>901-908</pages><issn>0041-0101</issn><eissn>1879-3150</eissn><coden>TOXIA6</coden><abstract>The bioactivity of Lophozozymus pictor toxin (LPTX) and the possible mechanism of action of the purified toxin are described. LPTX is found to possess palytoxin-like bioactivities. Besides exhibiting cytotoxic and haemolytic properties, LPTX causes the release of K + from erythrocytes and inhibits 2-[ 14C]deoxy-D-glucose uptake into HeLa cells. Although LPTX acts on HeLa cell and erythrocyte membranes, it does not interact with mitochondrial or liposomal membranes containing different phospholipid compositions. Ouabain, but not sphingomyelin, is able to prevent the toxic effects of LPTX. This antagonistic effect of ouabain on LPTX suggests that the toxin might mediate its toxic effects via the membrane Na + K + - ATPase but not through interaction with membrane lipids.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>8588214</pmid><doi>10.1016/0041-0101(95)00028-K</doi><tpages>8</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0041-0101
ispartof Toxicon (Oxford), 1995-07, Vol.33 (7), p.901-908
issn 0041-0101
1879-3150
language eng
recordid cdi_proquest_miscellaneous_16883086
source ScienceDirect Freedom Collection
subjects Animal poisons toxicology. Antivenoms
Animals
Biological and medical sciences
Brachyura - chemistry
HeLa Cells
Humans
Lophozozymus pictor
Marine
Marine Toxins - antagonists & inhibitors
Marine Toxins - chemistry
Marine Toxins - pharmacology
Medical sciences
Mitochondria, Liver - drug effects
Ouabain - pharmacology
Rats
Rats, Wistar
Sodium-Potassium-Exchanging ATPase - drug effects
Sphingomyelins - pharmacology
Toxicology
title Bioactivity and mechanism of action of Lophozozymus pictor toxin
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T12%3A33%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Bioactivity%20and%20mechanism%20of%20action%20of%20Lophozozymus%20pictor%20toxin&rft.jtitle=Toxicon%20(Oxford)&rft.au=Lau,%20C.O.&rft.date=1995-07-01&rft.volume=33&rft.issue=7&rft.spage=901&rft.epage=908&rft.pages=901-908&rft.issn=0041-0101&rft.eissn=1879-3150&rft.coden=TOXIA6&rft_id=info:doi/10.1016/0041-0101(95)00028-K&rft_dat=%3Cproquest_cross%3E16883086%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c417t-f283fd9ba400cabe8342a9ca4e0029c93675ae7d10f1d52258677ee4e8d9bcd43%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=16883086&rft_id=info:pmid/8588214&rfr_iscdi=true