Cloning of a locus involved in pathogenicity and production of extracellular polysaccharide and protease in Xanthomonas campestris pv. campestris

A Tn5‐induced mutant of Xanthomonas campestris pv campestris deficient in protease and extracellular polysaccharide production, and non‐pathogenic to turnip seedlings, was isolated. Recombinant clones which restored all three characteristics concomitantly were obtained from a wild‐type genomic libra...

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Bibliographic Details
Published in:FEMS microbiology letters 1994-03, Vol.117 (1), p.41-45
Main Authors: Rosato, Y.B., Destefano, S.A.L., Daniels, M.J.
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Bacterial plant pathogens
Biological and medical sciences
Extracellular polysaccharide
Fundamental and applied biological sciences. Psychology
Pathogenicity
Phytopathology. Animal pests. Plant and forest protection
Protease
Systematics. Structure, properties and multiplication. Genetics
Xanthomonas campestris
Xanthomonas campestris campestris
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Summary:A Tn5‐induced mutant of Xanthomonas campestris pv campestris deficient in protease and extracellular polysaccharide production, and non‐pathogenic to turnip seedlings, was isolated. Recombinant clones which restored all three characteristics concomitantly were obtained from a wild‐type genomic library. One of the plasmid clones, pIJ3090, showed no hybridization to previously isolated clones involved In protease production. The mutant was not complemented by other known clones involved in pathogenicity, in xanthan gum production or in protease production.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1994.tb06740.x