The importance of the fixation of colour, pattern and form in tropical Pseudocerotidae (Platyhelminthes, Polycladida)

At last a fixation method that ensures tropical pseudocerotid polyclads are fixed flat, preserved for histological preparation and which also retains their colour pattern has been developed. FCA-PGPP (Formaldehyde Calcium Acetate - Propylene Glycol, Propylene Phenoxetol) fixative is frozen and worms...

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Bibliographic Details
Published in:Hydrobiologia 1995, Vol.305 (1-3), p.141-143
Main Authors: NEWMAN, L. J, CANNON, L. R. G
Format: Article
Language:English
Subjects:
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
General aspects and techniques. Study of several systematic groups. Models
Invertebrates
Marine
Miscellaneous techniques
Pseudoceros
Techniques
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Summary:At last a fixation method that ensures tropical pseudocerotid polyclads are fixed flat, preserved for histological preparation and which also retains their colour pattern has been developed. FCA-PGPP (Formaldehyde Calcium Acetate - Propylene Glycol, Propylene Phenoxetol) fixative is frozen and worms are coaxed onto filter paper which is then laid on the frozen fixative. As a consequence, over 230 species have been documented from the southern Great Barrier Reef and eastern Papua New Guinea (Newman & Cannon, 1994; in press). It was determined that species diagnoses need to be based on colour pattern, general morphology of living animals and serial reconstructions of the male anatomy.
ISSN:0018-8158
1573-5117
DOI:10.1007/BF00036376