Production, characterization and application of monoclonal antibodies against the organophosphorus nerve agent Vx

Two monoclonal antibodies (Vx-BB8 and Vx-EA11) to the chemical warfare agent Vx were produced and characterized. A competitive inhibition enzyme immunoassay was developed to detect Vx concentrations as low as 3.7 x 10(-7) - 3.7 x 10(-6) mol/l in biological samples. Vx-BB8 400 micrograms given intrav...

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Bibliographic Details
Published in:Archives of toxicology 1995-08, Vol.69 (8), p.565-567
Main Authors: Ci, Y X, Zhou, Y X, Guo, Z Q, Rong, K T, Chang, W B
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - biosynthesis
Antibodies, Monoclonal - chemistry
Antibodies, Monoclonal - therapeutic use
Biological and medical sciences
Chemical and industrial products toxicology. Toxic occupational diseases
Chemical Warfare Agents - toxicity
Cholinesterase Inhibitors - immunology
Cholinesterase Inhibitors - toxicity
Female
Immunization, Passive
Immunoenzyme Techniques
Injections, Intraperitoneal
Injections, Intravenous
Medical sciences
Mice
Mice, Inbred BALB C
Organothiophosphorus Compounds - immunology
Organothiophosphorus Compounds - toxicity
Toxicology
Various organic compounds
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Summary:Two monoclonal antibodies (Vx-BB8 and Vx-EA11) to the chemical warfare agent Vx were produced and characterized. A competitive inhibition enzyme immunoassay was developed to detect Vx concentrations as low as 3.7 x 10(-7) - 3.7 x 10(-6) mol/l in biological samples. Vx-BB8 400 micrograms given intravenously immediately before 1 x LD95 Vx or 400 micrograms Vx-BB8 intraperitoneally 1.5 h-3 days before 1 x LD95 Vx could protect all the tested mice from death.
ISSN:0340-5761
1432-0738
DOI:10.1007/s002040050213