Development and clinical application of an InvaderPlus® assay for the detection of genital mycoplasmas

Abstract We developed a PCR-based assay involving Invader® technology for detection of the genital mycoplasmas of Mycoplasma genitalium , Mycoplasma hominis , Ureaplasma urealyticum , and Ureaplasma parvum . We compared its performance with that of a PCR-microtiter plate hybridization assay, which w...

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Bibliographic Details
Published in:Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy 2015-07, Vol.21 (7), p.516-519
Main Authors: Takanashi, Masaki, Ito, Shin, Kaneto, Hiroyuki, Tanahashi, Yoshikatsu, Kitanohara, Masataka, Yanagihara, Akira, Nakazima, Haruhiko, Yasuda, Mitsuru
Format: Article
Language:English
Subjects:
DNA, Bacterial - analysis
DNA, Bacterial - genetics
Hematology, Oncology and Palliative Medicine
Humans
Invader® technology
Male
Male Urogenital Diseases - diagnosis
Male Urogenital Diseases - microbiology
Molecular Typing - methods
Mycoplasma genitalium
Mycoplasma hominis
Mycoplasmataceae - genetics
Mycoplasmatales Infections - diagnosis
Mycoplasmatales Infections - microbiology
Polymerase Chain Reaction - methods
Ureaplasma parvum
Ureaplasma urealyticum
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Summary:Abstract We developed a PCR-based assay involving Invader® technology for detection of the genital mycoplasmas of Mycoplasma genitalium , Mycoplasma hominis , Ureaplasma urealyticum , and Ureaplasma parvum . We compared its performance with that of a PCR-microtiter plate hybridization assay, which we developed previously, in detecting genital mycoplasmas in first-voided urine (FVU) specimens from men with non-gonococcal urethritis. The tests targeting each of the genital mycoplasmas were specific for the respective species and could detect as few as 10 copies of the plasmids containing the target genes of each of the genital mycoplasmas per reaction. The assay using the InvaderPlus® method (InvaderPlus® assay) showed very similar performance to that of the PCR-microtiter plate hybridization assay for detecting the genital mycoplasmas in the FVU specimens. In addition, the PCR and endonuclease reaction in the InvaderPlus® assay were carried out simultaneously in one procedure, thus simplifying the assay, leading to time- and labor-savings and a decrease in the risk of specimen contamination. The InvaderPlus® assay could be useful in diagnosing genitourinary tract infections caused by the genital mycoplasmas.
ISSN:1341-321X
1437-7780
DOI:10.1016/j.jiac.2015.03.010