Biotype Diversity of Candida parapsilosis and Its Relationship to the Clinical Source and Experimental Pathogenicity

Environmental, vaginal, and blood isolates of Candida parapsilosis werebiotyped by karyotype analysis in pulsed-fieldgel electrophoresis. Morphotype and resistotype were also determined as was aspartyl proteinase secretion and pathogenicity in a systemic mouse infection model. Overall, the karyotype...

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Bibliographic Details
Published in:The Journal of infectious diseases 1995-04, Vol.171 (4), p.967-975
Main Authors: Cassone, A., Bernardis, F. De, Pontieri, E., Carruba, G., Girmenia, c., Martino, P., Fernández-Rodríguez, M., Quindós, G., Pontón, J.
Format: Article
Language:English
Subjects:
Animals
Aspartic Acid Endopeptidases - analysis
Blood
Candida - classification
Candida - genetics
Candida - pathogenicity
Candida albicans
Candida parapsilosis
Candidiasis - microbiology
Candidiasis, Vulvovaginal - microbiology
Central venous catheterization
Chromosomes
Chromosomes, Fungal
Electrophoresis, Gel, Pulsed-Field
Female
Fungemia - microbiology
Humans
Infections
Karyotype
Karyotyping
Major Articles
Mice
Mortality
Mycological Typing Techniques
Neutropenia - chemically induced
Opportunistic Infections - microbiology
Soil Microbiology
Vagina - microbiology
Vaginitis
Virulence
Yeasts
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Summary:Environmental, vaginal, and blood isolates of Candida parapsilosis werebiotyped by karyotype analysis in pulsed-fieldgel electrophoresis. Morphotype and resistotype were also determined as was aspartyl proteinase secretion and pathogenicity in a systemic mouse infection model. Overall, the karyotype patterns consisted of 6-9 chromosome bands (>3.0-0.6 Mb) with limited clustering, since most isolates had unique chromosome profiles. Major clusters of C. parapsilosis, differing by source of isolation and in experimental pathogenicity, could be discriminated by morphoresistotyping. The morphotypes of isolates from subjects with candidemia ranged from one that caused elevated mortality in the normal mouse to those that were totally avirulent in the neutropenic animal. Among clinical isolates, secretion of aspartyl proteinase washigher in vaginitis than in candidemia isolates and did not correlate with the experimental pathogenicity. These results emphasize the biotype diversity of C. parapsilosis and have potentially important epidemiologic and pathologic implications.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/171.4.967