Preparation, composition analysis and antioxidant activities of konjac oligo-glucomannan

•Konjac oligo-glucomannan was prepared by degradation of konjac glucomannan.•The average degree of polymerization of the konjac oligo-glucomannan was 5.2.•FTIR spectra indicated that konjac glucomannan was successfully degraded.•KOG exhibited significant antioxidant activities but weaker than Vc. Ko...

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Bibliographic Details
Published in:Carbohydrate polymers 2015-10, Vol.130, p.398-404
Main Authors: Liu, Jianhua, Xu, Qiuhong, Zhang, Jingjing, Zhou, Xuxia, Lyu, Fei, Zhao, Peicheng, Ding, Yuting
Format: Article
Language:English
Subjects:
Amorphophallus - chemistry
Antioxidant activity
Antioxidants - chemistry
Antioxidants - pharmacology
beta-Mannosidase - metabolism
Biphenyl Compounds - chemistry
Composition
Hydrolysis
Hydroxyl Radical - chemistry
Konjac glucomannan (KG)
Konjac oligo-glucomannan (KOG)
Mannans - chemistry
Mannans - pharmacology
Oxidation-Reduction
Picrates - chemistry
Spectroscopy, Fourier Transform Infrared
β-Mannanase
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Summary:•Konjac oligo-glucomannan was prepared by degradation of konjac glucomannan.•The average degree of polymerization of the konjac oligo-glucomannan was 5.2.•FTIR spectra indicated that konjac glucomannan was successfully degraded.•KOG exhibited significant antioxidant activities but weaker than Vc. Konjac oligo-glucomannan (KOG) was prepared by degradation of konjac glucomannan (KG) using β-mannanase. The hydrolysis process was monitored by the viscosity of the enzymatic hydrolysates. Factors affecting the enzymatic hydrolysis of KG were investigated, and the optimum hydrolysis conditions were as follows: time 2h; temperature 50°C; pH 6.0; and enzymatic concentration 150U/g. Under these optimized conditions, minimum viscosity (31.9mPa·s) of the hydrolasate was obtained. The average degree of polymerization (DP) of the resulting KOG was approximately equal to 5.2. The results of Fourier transform infrared (FTIR) spectra of KG and KOG indicated that KG was successfully degraded. In addition, their antioxidant activities were evaluated by determination of hydroxyl radical (•OH) and 1,1-diphenyl-2-picrylhrazyl radical (•DPPH) scavenging activity, and determination of reducing power. The results showed that KOG exhibited significant antioxidant activities. Taken together, this study suggested that KOG could potentially be used as a natural antioxidant.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2015.05.025