Structural characterization of a polysaccharide from Chrysanthemum morifolium flowers and its antioxidant activity

• Published in: Carbohydrate polymers 2015-10, Vol.130, p.113-121
• Main Authors: Zheng, Changping, Dong, Qun, Chen, Huanjun, Cong, Qifei, Ding, Kan
• Format: Article
• Language: English
• Subjects: Animals; Antioxidant; Antioxidants - chemistry; Antioxidants - pharmacology; Carbohydrate Conformation; Carbohydrate Sequence; Chrysanthemum - chemistry; Chrysanthemum morifolium; Flowers - chemistry; Hydrogen Peroxide - pharmacology; Hydrolysis; Magnetic Resonance Spectroscopy; Molecular Sequence Data; Molecular Weight; Monosaccharides - analysis; PC12 Cells - drug effects; Polysaccharide; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Rats; Spectrometry, Mass, Electrospray Ionization; Spectroscopy, Fourier Transform Infrared; Structural elucidation
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2015.05.004

•A novel polysaccharide CMJA0S2 was isolated and purified from Chrysanthemum morifolium flowers.•The structure of CMJ0S2 formed with a manogalactoglucan backbone was elucidated.•The polysaccharide CMJA0S2 exhibited significant anti-oxidative effect. The flowers of Chrysanthemum morifolium were extracted by boiling water, and a water-soluble polysaccharide (CMJA0S2) with a molecular weight of 6.5kDa was isolated by anion-exchange chromatography on a DEAE-cellulose column and gel permeation chromatography on a Sephacryl S-300 HR column. Monosaccharide composition analysis indicated that CMJA0S2 was composed of galactose, glucose, mannose and arabinose in molar ratio of 4.1: 3.3: 1.0: 2.3. According to linkage analysis, partial acid hydrolysis and NMR spectra, the backbone was shown to contain 1, 4-linked β-Galp, 1, 4-linked β-Glcp and 1, 4-linked β-Manp, with branches substituted at C-6 of 1, 4-linked β-Manp by 1, 6-linked β-Galp and at O-6 of partial 1, 4-linked β-Galp substituted by T-linked α-Glcp. About 40% of 1, 6-linked β-Galp with T-linked α-Glcp was substituted at O-3 by α-Araf-(1→[5)-α-Araf-(1]3. The anti-oxidative analysis showed that CMJA0S2 could scavenge the DPPH radicals and relieve the damage of PC12 cells caused by H2O2, thus it could be regarded as a potential natural antioxidant.