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Isolation, identification and differentiation of human embryonic cartilage stem cells

We isolated human embryonic cartilage stem cells (hECSCs), a novel stem cell population, from the articular cartilage of eight‐week‐old human embryos. These stem cells demonstrated a marker expression pattern and differentiation potential intermediate to those of human embryonic stem cells (hESCs) a...

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Published in:Cell biology international 2015-07, Vol.39 (7), p.777-787
Main Authors: Fu, Changhao, Yan, Zi, Xu, Hao, Zhang, Chen, Zhang, Qi, Wei, Anhui, Yang, Xi, Wang, Yi
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container_title Cell biology international
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creator Fu, Changhao
Yan, Zi
Xu, Hao
Zhang, Chen
Zhang, Qi
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Yang, Xi
Wang, Yi
description We isolated human embryonic cartilage stem cells (hECSCs), a novel stem cell population, from the articular cartilage of eight‐week‐old human embryos. These stem cells demonstrated a marker expression pattern and differentiation potential intermediate to those of human embryonic stem cells (hESCs) and human adult stem cells (hASCs). hECSCs expressed markers associated with both hESCs (OCT4, NANOG, SOX2, SSEA‐3 and SSEA‐4) and human adult stem cells (hASCs) (CD29, CD44, CD90, CD73 and CD10). These cells also differentiated into adipocytes, osteoblasts, chondrocytes, neurons and islet‐like cells under specific inducing conditions. We identified N6, 2′‐O‐dibutyryl cyclic adenosine 3′:5′‐monophosphate (Bt2cAMP) as an inducer of chondrogenic differentiation in hECSCs. Similar results using N6, 2′‐O‐ dibutyryl cyclic adenosine 3′:5′‐ monophosphate (Bt2cAMP) were obtained for two other types of human embryonic tissue‐derived stem cells, human embryonic hepatic stem cells (hEHSCs) and human embryonic amniotic fluid stem cells (hEASCs), both of which exhibited a marker expression pattern similar to that of hECSCs. The isolation of hECSCs and the discovery that N6, 2′‐O‐ dibutyryl cyclic adenosine 3′:5′‐ monophosphate (Bt2cAMP) induces chondrogenic differentiation in different stem cell populations might aid the development of strategies in tissue engineering and cartilage repair.
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subjects Adenosine
Adipocytes - cytology
articular cartilage
Bone marrow
Bt2cAMP
Cartilage, Articular - cytology
Cell Differentiation
Cell Separation - methods
Chondrocytes - cytology
chondrogenesis
Chondrogenesis - drug effects
Cyclic AMP - analogs & derivatives
Cyclic AMP - pharmacology
Embryonic Stem Cells - cytology
Embryonic Stem Cells - drug effects
Embryos
expression of markers
Female
human embryonic cartilage stem cells (hECSCs)
Humans
Islets of Langerhans - cytology
Stem cells
Tissue Engineering
title Isolation, identification and differentiation of human embryonic cartilage stem cells
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