Functional role and mechanism of lncRNA LOC728228 in malignant 16HBE cells transformed by anti-benzopyrene-trans-7,8-dihydrodiol-9,10-epoxide

Lung cancer is a major health problem, and is considered one of the deadliest cancers in humans. It is refractory to current treatments, and the mechanisms of lung cancer are unknown. Long noncoding RNAs (lncRNAs) are involved in various biological processes and human diseases. However, the exact fu...

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Published in:Molecular carcinogenesis 2015-06, Vol.54 (S1), p.E192-E204
Main Authors: Hu, Gongcheng, Yang, Ti, Zheng, Jingli, Dai, Jiabing, Nan, Aruo, Lai, Yandong, Zhang, Yajie, Yang, Chengfeng, Jiang, Yiguo
Format: Article
Language:English
Subjects:
16HBE-T cells
7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide - toxicity
Animals
Cell Line, Transformed
Humans
LOC728228
long noncoding RNA
lung cancer
Lung Neoplasms - pathology
Mice
Mice, Nude
Polymerase Chain Reaction
RNA, Long Noncoding - physiology
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Summary:Lung cancer is a major health problem, and is considered one of the deadliest cancers in humans. It is refractory to current treatments, and the mechanisms of lung cancer are unknown. Long noncoding RNAs (lncRNAs) are involved in various biological processes and human diseases. However, the exact functional roles and mechanisms of lncRNAs are largely unclear. In this study, we attempted to identify lung‐cancer‐related lncRNAs. We found changes in lncRNA expression in the anti‐benzo(a) pyrene‐7,8‐diol‐9,10‐epoxide (anti‐BPDE)‐transformed human bronchial epithelial cell line (16HBE‐T cells) using microarrays and qRT–PCR. Of these lncRNAs, LOC728228 was upregulated relative to its expression in control untransformed16HBE (16HBE‐N) cells. LOC728228 knockdown inhibited cell proliferation, caused G0/G1‐phase cell‐cycle arrest, reduced cellular migration, suppressed colony formation in vitro, and inhibited tumor growth in a nude mouse xenograft model. LOC728228 knockdown also suppressed cyclin D1 expression, and the depletion of cyclin D1 induced G0/G1‐phase cell‐cycle arrest and inhibited cell proliferation, thus influencing the malignant potential of cancer cells. In summary, our results suggest that lncRNA LOC728228 has an oncogene‐like function and plays a vital role in human lung cancer. © 2015 Wiley Periodicals, Inc.
ISSN:0899-1987
1098-2744
DOI:10.1002/mc.22314