Epidemiology of canine leishmaniasis in southern Bahia, Brazil

• Published in: Acta tropica 2015-08, Vol.148, p.115-119
• Main Authors: Leça Júnior, Nilo Fernandes, Guedes, Paula Elisa Brandão, Santana, Lailla Nascimento, Almeida, Valter dos Anjos, Carvalho, Fábio Santos, Albuquerque, George Rego, Wenceslau, Amauri Arias, Munhoz, Alexandre Dias, Silva, Fabiana Lessa
• Format: Article
• Language: English
• Subjects: Animals; Asymptomatic Infections; Brazil - epidemiology; Dog Diseases - epidemiology; Dog Diseases - parasitology; Dogs; Fluorescent Antibody Technique, Indirect; Indirect immunofluorescence; Leishmania - genetics; Leishmania sp; Leishmaniasis, Cutaneous - epidemiology; Leishmaniasis, Cutaneous - parasitology; Leishmaniasis, Cutaneous - veterinary; Leishmaniasis, Visceral - epidemiology; Leishmaniasis, Visceral - parasitology; Leishmaniasis, Visceral - veterinary; Polymerase Chain Reaction; Prevalence; Risk factors
• ISSN: 0001-706X; 1873-6254
• DOI: 10.1016/j.actatropica.2015.04.008

•An epidemiological study of canine leishmaniosis was conducted in Buerarema, Bahia.•A high prevalence of infection by Leishmania sp. (50.3%) was detected by IFA.•All the dogs were negative for Leishmania infantum chagasi infection by PCR.•Most dogs infected with Leishmania braziliensis from Buerarema are asymptomatic.•The detection sensitivity of the IFA was higher than that of the PCR. Leishmaniosis is a zoonosis caused by protozoa of the genus Leishmania. American cutaneous leishmaniosis (ACL) is mainly caused by the species L. amazonensis and L. braziliensis, and American visceral leishmaniosis (AVL) is caused by L. infantum chagasi. In addition to their proven roles as reservoirs of AVL, dogs are also suspected by researchers to be reservoirs of ACL due to reports of this infection in domestic environments and of infected dogs in endemic areas. The aim of this study was to detect Leishmania sp. infection in dogs from Vila Operária, Buerarema, Bahia, using parasitological tests, indirect immunofluorescent assay (IFA) and polymerase chain reaction (PCR). Furthermore, this study also aimed to identify risk factors associated with illness in dogs in this locality by conducting an epidemiological survey. For this purpose, 292 dogs were clinically evaluated for the presence of skin lesions, and the dogs that showed these changes were submitted to scarification injury to enable preparation of slides for microscopic study of amastigotes. Subsequently, the dogs underwent blood sampling for serological (IFA) and molecular (PCR) tests. Additionally, the owners of the dogs answered an epidemiological questionnaire to facilitate the identification of risk factors for exposure of dogs to pathogens of ACL. Of the 292 dogs studied, 13 (4.5%) had lesions suggestive of ACL, but with a negative parasitological examination and 147 (50.3%) were seropositive according to the IFA. Of the 273 dogs studied using PCR test, 10 (3.66%) were positive for L. braziliensis, and all samples were negative for L. infantum chagasi. Wastelands in the peridomicile and the presence of light in the household were risk factors associated with ACL. The results show that Vila Operária has asymptomatic dogs with ACL and that the detection sensitivity of the IFA was higher than that of PCR for the infected dogs.