Quantification of 2,4-D and related chlorophenoxy herbicides by a magnetic particle-based ELISA

The development of a magnetic particle based ELISA for 2,4-D is described. The assay sensitivity, based on the concentration producing 90 per cent of the light absorbance at 450 nm of a zero standard was 0.7 ppb. Recovery of a reference standard, was an average of 98 per cent across a concentration...

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Bibliographic Details
Published in:Bulletin of environmental contamination and toxicology 1994-04, Vol.52 (4), p.538-545
Main Authors: Lawruk, T.S, Hottenstein, C.S, Fleeker, J.R, Hall, J.C, Herzog, D.P, Rubio, F.M
Format: Article
Language:English
Subjects:
2,4-D
2,4-Dichlorophenoxyacetic Acid - analysis
AGUA
ANALISIS CUANTITATIVO
ANALYSE QUANTITATIVE
Analytical chemistry
Chemistry
Contamination
Desorption
EAU
ELISA
Enzyme-Linked Immunosorbent Assay - methods
ESTER
ESTERES
ESTERS
Exact sciences and technology
HERBICIDAS
HERBICIDE
HERBICIDES
Herbicides - analysis
Miscellaneous
POLLUTION DE L'EAU
POLUCION DEL AGUA
Q1
QUANTITATIVE ANALYSIS
Runoff
Sensitivity and Specificity
TEST ELISA
WATER
Water Pollutants, Chemical - analysis
WATER POLLUTION
Water resources
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Description
Summary:The development of a magnetic particle based ELISA for 2,4-D is described. The assay sensitivity, based on the concentration producing 90 per cent of the light absorbance at 450 nm of a zero standard was 0.7 ppb. Recovery of a reference standard, was an average of 98 per cent across a concentration range, and the accuracy was linear. Groundwater samples assayed by this ELISA and an established gas chromatography/mass spectroscopy method gave a correlation of 0.970 and a slope of 0.938 between methods. Specificity data for a variety of chlorophenoxy analogues and unrelated chemicals showed that the ELISA was up to 20 times more sensitive to esters of 2,4-D. Other groundwater components did not interfere with the assay at concentrations higher than usually found. The assay was fast, sensitive and accurate and appeared suitable for monitoring 2,4-D in water.
ISSN:0007-4861
1432-0800
DOI:10.1007/BF00194141