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Parthenogenetic activation of oocytes in c- mos -deficient mice
IN Xenopus the c- mos proto-oncogene product (Mos) is essential for the initiation of oocyte maturation 1 , for the progression from meiosis I to meiosis II 2,3 and for the second meiotic metaphase arrest, acting as an essential component of the cytostatic factor CSF 4,5 . Its function in mouse oocy...
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Published in: | Nature (London) 1994-07, Vol.370 (6484), p.68-71 |
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Main Authors: | , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | IN
Xenopus
the c-
mos
proto-oncogene product (Mos) is essential for the initiation of oocyte maturation
1
, for the progression from meiosis I to meiosis II
2,3
and for the second meiotic metaphase arrest, acting as an essential component of the cytostatic factor CSF
4,5
. Its function in mouse oocytes is unclear
6–9
, however, as is the biological significance of c-
mos
mRNA expression in testes
1,10
and several somatic tissues
1,10,11
. We have generated c-
mos
-deficient mice by gene targeting in embryonic stem cells. These mice grew at the same rate as their wild-type counterparts and reproduction was normal in the males, but the fertility of the females was very low. The c-
mos
-deficient female mice developed ovarian teratomas at a high frequency. Oocytes from these females matured to the second meiotic metaphase both
in vivo
and
in vitro
, but were activated without fertilization. The results indicate that in mice Mos plays a role in the second meiotic metaphase arrest, but does not seem to be essential for the initiation of oocyte maturation, spermatogenesis or somatic cell cycle. |
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ISSN: | 0028-0836 1476-4687 |
DOI: | 10.1038/370068a0 |