Loading…

Parthenogenetic activation of oocytes in c- mos -deficient mice

IN Xenopus the c- mos proto-oncogene product (Mos) is essential for the initiation of oocyte maturation 1 , for the progression from meiosis I to meiosis II 2,3 and for the second meiotic metaphase arrest, acting as an essential component of the cytostatic factor CSF 4,5 . Its function in mouse oocy...

Full description

Saved in:
Bibliographic Details
Published in:Nature (London) 1994-07, Vol.370 (6484), p.68-71
Main Authors: Hashimoto, Naohiro, Watanabe, Nobumoto, Furuta, Yasuhide, Tamemoto, Hiroyuki, Sagata, Noriyuiki, Yokoyama, Minesuke, Okazaki, Kenji, Nagayoshi, Mariko, Takedat, Naoki, Ikawatll, Yoji, Aizawai, Shinichi
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:IN Xenopus the c- mos proto-oncogene product (Mos) is essential for the initiation of oocyte maturation 1 , for the progression from meiosis I to meiosis II 2,3 and for the second meiotic metaphase arrest, acting as an essential component of the cytostatic factor CSF 4,5 . Its function in mouse oocytes is unclear 6–9 , however, as is the biological significance of c- mos mRNA expression in testes 1,10 and several somatic tissues 1,10,11 . We have generated c- mos -deficient mice by gene targeting in embryonic stem cells. These mice grew at the same rate as their wild-type counterparts and reproduction was normal in the males, but the fertility of the females was very low. The c- mos -deficient female mice developed ovarian teratomas at a high frequency. Oocytes from these females matured to the second meiotic metaphase both in vivo and in vitro , but were activated without fertilization. The results indicate that in mice Mos plays a role in the second meiotic metaphase arrest, but does not seem to be essential for the initiation of oocyte maturation, spermatogenesis or somatic cell cycle.
ISSN:0028-0836
1476-4687
DOI:10.1038/370068a0