Biodegradation of a colloidal ester-based polyurethane by soil fungi

Four species of fungi were isolated from soil and found to degrade ester-based polyurethane in a polyurethane-agar clearing assay. One of these isolates, Curvularia senegalensis, secreted an extracellular enzyme-like factor with esterase properties, as determined by fluorescein diacetate hydrolysis,...

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Bibliographic Details
Published in:International biodeterioration & biodegradation 1994, Vol.33 (2), p.103-113
Main Authors: Crabbe, Joel R., Campbell, James R., Thompson, Laura, Walz, Stephen L., Schultz, Warren W.
Format: Article
Language:English
Subjects:
Biodegradation of pollutants
Biological and medical sciences
Biotechnology
Environment and pollution
Fundamental and applied biological sciences. Psychology
Industrial applications and implications. Economical aspects
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Summary:Four species of fungi were isolated from soil and found to degrade ester-based polyurethane in a polyurethane-agar clearing assay. One of these isolates, Curvularia senegalensis, secreted an extracellular enzyme-like factor with esterase properties, as determined by fluorescein diacetate hydrolysis, and this active factor was partially purified by ammonium sulfate fractionation. The polyurethane-clearing factor had an isoelectric point of 5·1 and a molecular weight of 28 kDa. It was stable to heating at 100°C for 10 min and was inhibited by phenylmethylsulfonyl fluoride.
ISSN:0964-8305
1879-0208
DOI:10.1016/0964-8305(94)90030-2