Suitability of aqueous dispersions of dextran and Concanavalin A for glucose sensing in different variants of the affinity sensor

Aqueous dispersions of dextran and Concanavalin A (Con A) were investigated for possible application as the essential part of a glucose affinity sensor. The considered variants of signal formation were based on the sugar-dependent dissociation of Con A and dextran, which were enclosed in a small dia...

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Bibliographic Details
Published in:Biosensors & bioelectronics 1994, Vol.9 (8), p.557-567
Main Authors: Ballerstädt, R., Ehwald, R.
Format: Article
Language:English
Subjects:
affinity sensor
Biological and medical sciences
Biosensors
Biotechnology
Concanavalin A
dextran
Fundamental and applied biological sciences. Psychology
glucose
Methods. Procedures. Technologies
Sephadex
Various methods and equipments
viscosity
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Summary:Aqueous dispersions of dextran and Concanavalin A (Con A) were investigated for possible application as the essential part of a glucose affinity sensor. The considered variants of signal formation were based on the sugar-dependent dissociation of Con A and dextran, which were enclosed in a small dialysis chamber with a membrane permeable to sugars but not to macromolecules. Two alternative proposals were investigated: (a) determination of the concentration of dissolved labelled lectin in equilibrium with a lectin fraction bound to cross-linked dextran or (b) determination of the viscosity of an homogeneous dextran-lectin dispersion. For both approaches, suitable conditions were established to give a strong dependence of the measured parameter on the glucose concentration in a concentration range relevant for blood sugar sensing. Both glucose-dependent Con A distribution and glucose-dependent viscosity changes of the dextran/Con A solution showed a sufficiently fast response (
ISSN:0956-5663
1873-4235
DOI:10.1016/0956-5663(94)80048-0