Expression of melatonin receptors genes and genes associated with regulation of their activity in endometrial cancer

The aim of the study was to evaluate transcription activity of melatonin receptors and genes associated with regulation of their activity in endometrial adenocarcinoma to identify probable diagnostic and prognostic molecular markers. The material included endometrial adenocarcinoma tissue samples of...

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Published in:Ginekologia polska 2015-01, Vol.86 (4), p.248-255
Main Authors: Witek, Andrzej, Jęda, Agnieszka, Baliś, Michał, Orchel, Joanna, Sikora, Bartosz, Skubis, Aleksandra, Szota-Czyż, Justyna, Janikowski, Tomasz, Mazurek, Urszula
Format: Article
Language:eng ; pol
Subjects:
Carcinoma, Endometrioid - genetics
Carcinoma, Endometrioid - metabolism
Down-Regulation
Endometrial cancer
Endometrial Neoplasms - genetics
Endometrial Neoplasms - metabolism
Endometrium
Female
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Humans
Melatonin
Real-Time Polymerase Chain Reaction
Receptors, Melatonin - genetics
Receptors, Melatonin - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
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container_issue 4
container_start_page 248
container_title Ginekologia polska
container_volume 86
creator Witek, Andrzej
Jęda, Agnieszka
Baliś, Michał
Orchel, Joanna
Sikora, Bartosz
Skubis, Aleksandra
Szota-Czyż, Justyna
Janikowski, Tomasz
Mazurek, Urszula
description The aim of the study was to evaluate transcription activity of melatonin receptors and genes associated with regulation of their activity in endometrial adenocarcinoma to identify probable diagnostic and prognostic molecular markers. The material included endometrial adenocarcinoma tissue samples of histopathological grades G1, G2, G3, and normal endometrium. The molecular analysis was performed on 37 patient samples. Total RNA was extracted and used for the microarray HG-U133A analysis. Among 22 283 ID mRNA, only entities of genes associated with regulation of melatonin receptors activity were selected. qRT-PCR was employed for validation, what allowed to compare melatonin receptor genes activation in endometrial cancer tissues to the normal endometrium. The results of the microarray experiments showed that only 18 ID mRNA were differential in endometrial cancer samples as compared to the control at p-value1.5. These genes were identified as differentially expressed in grade G2-ASMTL, GNA 11, PER2, PTGDS and in grade G3-GNA12, GNA 11. Silencing of RGS4 encoding RGP4, which regulates signal transmission by G protein, was observed in all cancer groups, independently of the histopathological grade. The profile expression of genes associated with regulation of melatonin receptors activity was different and dependent on the histopathological grade of endometrial cancer and can be an additional diagnostic and prognostic marker Statistically significant was the down-regulation of melatonin biosynthesis genes (ASMTL) and melatonin signal transmitters (GNA 11, GNA 12, RTGS).
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subjects Carcinoma, Endometrioid - genetics
Carcinoma, Endometrioid - metabolism
Down-Regulation
Endometrial cancer
Endometrial Neoplasms - genetics
Endometrial Neoplasms - metabolism
Endometrium
Female
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Humans
Melatonin
Real-Time Polymerase Chain Reaction
Receptors, Melatonin - genetics
Receptors, Melatonin - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
title Expression of melatonin receptors genes and genes associated with regulation of their activity in endometrial cancer
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