Mechanism of the uptake of cationic and anionic calcium phosphate nanoparticles by cells

The uptake of calcium phosphate nanoparticles (diameter 120nm) with different charge by HeLa cells was studied by flow cytometry. The amount of uptaken nanoparticles increased with increasing concentration of nanoparticles in the cell culture medium. Several inhibitors of endocytosis and macropinocy...

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Bibliographic Details
Published in:Acta biomaterialia 2013-07, Vol.9 (7), p.7527-7535
Main Authors: Sokolova, Viktoriya, Kozlova, Diana, Knuschke, Torben, Buer, Jan, Westendorf, Astrid M., Epple, Matthias
Format: Article
Language:English
Subjects:
Anions
Biotechnology
Calcium phosphate
calcium phosphates
Calcium Phosphates - chemistry
Cationic
Cations
cell culture
Cell Membrane - chemistry
Chlorpromazine
culture media
Endocytosis
Flow cytometry
Hydrogen-Ion Concentration
Inhibitors
lysosomes
Nanoparticles
Nanoparticles - chemistry
Nanoparticles - ultrastructure
nystatin
Particle Size
Transport Vesicles - chemistry
Uptakes
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Summary:The uptake of calcium phosphate nanoparticles (diameter 120nm) with different charge by HeLa cells was studied by flow cytometry. The amount of uptaken nanoparticles increased with increasing concentration of nanoparticles in the cell culture medium. Several inhibitors of endocytosis and macropinocytosis were applied to elucidate the uptake mechanism of nanoparticles into HeLa cells: wortmannin, LY294002, nocodazole, chlorpromazine and nystatin. Wortmannin and LY294002 strongly reduced the uptake of anionic nanoparticles, which indicates macropinocytosis as uptake mechanism. For cationic nanoparticles, the uptake was reduced to a lesser extent, indicating a different uptake mechanism. The localization of nanoparticles inside the cells was investigated by conjugating them with the pH-sensitive dye SNARF-1. The nanoparticles were localized in lysosomes after 3h of incubation.
ISSN:1742-7061
1878-7568
DOI:10.1016/j.actbio.2013.02.034