PLA-HA Scaffolds: Preparation and Bioactivity

The photocurable polylactide (PLA) and hydroxyapatite (HA) composites were prepared for solid free-form fabrication of three-dimensional scaffolds by stereolithography (SLA). The influence of varying pore feature and HA amount of PLA/HA scaffolds on cell growth and differentiation was evaluated with...

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Bibliographic Details
Published in:Procedia engineering 2013, Vol.59, p.144-149
Main Authors: Tanodekaew, Siriporn, Channasanon, Somruethai, Kaewkong, Pakkanun, Uppanan, Paweena
Format: Article
Language:English
Subjects:
bioactivity
Biochemistry
Biomedical materials
Culture
Differentiation
Hydroxyapatite
polylactide
Pore size
Porosity
scaffold
Scaffolds
stereolithography
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Summary:The photocurable polylactide (PLA) and hydroxyapatite (HA) composites were prepared for solid free-form fabrication of three-dimensional scaffolds by stereolithography (SLA). The influence of varying pore feature and HA amount of PLA/HA scaffolds on cell growth and differentiation was evaluated with MC3T3-E1 pre-osteoblasts. Morphology of adherent cells on scaffolds was examined by SEM. Alamar Blue assay was employed to observe proliferation and cytotoxicity of cells on scaffold. Alkaline phosphatase activity (ALP) and calcium content in cell-cultured samples were also measured to assess osteoblast activity. The cellular responses to various types of scaffolds at a 21-day culture period were found similar while the ALP activity observed at a 7-day culture period appeared to be superior in the scaffold with smaller pore size and higher amount of HA. These results indicated that HA and pore size had an effect on bioactivity of scaffold only at the early state of osteoblastic differentiation. At the longer culture period, all types of scaffolds showed equally cell proliferation and function, and thus demonstrated their potential to be used in bone repair application.
ISSN:1877-7058
1877-7058
DOI:10.1016/j.proeng.2013.05.104